Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Regulation of Tn5 transposition in Salmonella typhimurium.

Literature DB >> 6255475

Regulation of Tn5 transposition in Salmonella typhimurium.

Abstract

The drug-resistance element Tn5 transposes with high frequency immediately after entry into a cell. Establishment of Tn5 within a cell results in a decrease in this transposition frequency. This phenomenon resembles "zygotic induction" of repressible operons and prophages. Evidence is presented that Tn5 transposition is under negative control by a factor encoded within the element itself. Established Tn5 elements (that contain point mutations inactivating the resistance gene) are able to inhibit transposition of an incoming Tn5 element by a factor of 12- to 70-fold. Several deletion derivatives of Tn5 lack the ability to inhibit transposition.

Entities: Species

Mesh：

Substances：

Year: 1980 PMID： 6255475 PMCID： PMC350210 DOI： 10.1073/pnas.77.10.6047

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

19 in total

Regulation of Tn5 transposition in Salmonella typhimurium.

1. Gene duplication in bacteria: alteration of gene dosage by sister-chromosome exchanges.

2. In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites.

3. Analysis of sequences transposed by complementation of two classes of transposition-deficient mutants of Tn3.

4. Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance element.

5. Translocatable elements in procaryotes.

6. Phage P22-mutants with increased or decreased transduction abilities.

7. Inhibition of TnA translocation by TnA.

8. Localized mutagenesis of any specific small region of the bacterial chromosome.

9. Procedure for identifying nonsense mutations.

10. Transposition of R factor genes to bacteriophage lambda.

1. Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequences.

2. Transposon mutagenesis affecting thiosulfate oxidation in Bosea thiooxidans, a new chemolithoheterotrophic bacterium.

3. Genetic and physical mapping of the mcrA (rglA) and mcrB (rglB) loci of Escherichia coli K-12.

4. Temporal control of transposition in Tn5.

5. recA-independent recombination between repeated IS50 elements is not caused by an IS50-encoded function.

6. p2 and inhibition of Tn5 transposition.

7. Activation of silent genes by transposons Tn5 and Tn10.

8. Characterization of the Tn5 transposase and inhibitor proteins: a model for the inhibition of transposition.

9. Mutagenesis of Alcaligenes eutrophus by insertion of the drug-resistance transposon Tn5.

10. DHA system mediating aerobic and anaerobic dissimilation of glycerol in Klebsiella pneumoniae NCIB 418.