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Literature DB >> 6276674

Apurinic DNA endonucleases from Drosophila melanogaster embryos.

Abstract

Apurinic DNA endonuclease activity from Drosophila melanogaster embryos was resolved into two separable forms by phosphocellulose chromatography, one which flowed through the column (Fraction I) and the other which was retained and eluted at approximately 200 mM potassium phosphate (Fraction II). Both fractions, purified further by glycerol gradient sedimentation, were found to introduce nicks into DNA that were specific for and equal in number to the alkali-labile sites in depurinated DNA. They had similar apparent Km values for apurinic sites (0.7 nM apurinic sites for Fraction I and 0.8 nM for Fraction II), but differed with respect to optimal pH, Mg++ requirement and sensitivity to EDTA.

Entities: Chemical Species

Mesh：

Substances：
DNA
Endonucleases

Year: 1981 PMID： 6276674 DOI： 10.1007/bf00270157

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

13 in total

1. Statistical estimations in enzyme kinetics.

Authors: G N WILKINSON
Journal: Biochem J Date: 1961-08 Impact factor: 3.857

2. An altered apurinic DNA endonuclease activity in group A and group D xeroderma pigmentosum fibroblasts.

Authors: U Kuhnlein; E E Penhoet; S Linn
Journal: Proc Natl Acad Sci U S A Date: 1976-04 Impact factor: 11.205

3. DNA binding activity from cultured human fibrolasts that is specific for partially depurinated DNA and that inserts purines into apurinic sites.

Authors: W A Deutsch; S Linn
Journal: Proc Natl Acad Sci U S A Date: 1979-01 Impact factor: 11.205

4. An endonuclease from Escherichia coli that introduces single polynucleotide chain scissions in ultraviolet-irradiated DNA.

Authors: M Radman
Journal: J Biol Chem Date: 1976-03-10 Impact factor: 5.157

5. Properties of the main endonuclease specific for apurinic sites of Escherichia coli (endonuclease VI). Mechanism of apurinic site excision from DNA.

Authors: F Gossard; W G Verly
Journal: Eur J Biochem Date: 1978-01-16

6. DNA glycosylases, endonucleases for apurinic/apyrimidinic sites, and base excision-repair.

Authors: T Lindahl
Journal: Prog Nucleic Acid Res Mol Biol Date: 1979

7. Human endonuclease specific for apurinic/apyrimidinic sites in DNA. Partial purification and characterization of multiple forms from placenta.

Authors: W S Linsley; E E Penhoet; S Linn
Journal: J Biol Chem Date: 1977-02-25 Impact factor: 5.157

8. Endonuclease from Escherichia coli that acts specifically upon duplex DNA damaged by ultraviolet light, osmium tetroxide, acid, or x-rays.

Authors: F T Gates; S Linn
Journal: J Biol Chem Date: 1977-05-10 Impact factor: 5.157

4. Drosophila Rrp1 complements E. coli xth nfo mutants: protection against both oxidative and alkylation-induced DNA damage.

Authors: L Gu; S M Huang; M Sander
Journal: Nucleic Acids Res Date: 1993-10-11 Impact factor: 16.971

5. Repair of alkylated DNA: Drosophila have DNA methyltransferases but not DNA glycosylases.

Authors: D A Green; W A Deutsch
Journal: Mol Gen Genet Date: 1983

6. Characterization of a depurinated-DNA purine-base-insertion activity from Drosophila.

Authors: W A Deutsch; A L Spiering
Journal: Biochem J Date: 1985-11-15 Impact factor: 3.857

6 in total