Literature DB >> 6276443

Alkaline phosphatase for immunocytochemical labelling: problems with endogenous enzyme activity.

A S Bulman, E Heyderman.   

Abstract

Alkaline phosphatase may be used as a label for immunocytochemistry and can be demonstrated in tissue sections using the single step naphthol phosphate method. Endogenous enzyme activity may not be destroyed by fixation in formalin, formol alcohol, Carnoy's or Baker's solutions and should be inhibited before results are assessed. Either Bouin's solution or periodic acid followed by potassium borohydride are satisfactory inhibitor and do not adversely affect immunocytochemical results.

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Year:  1981        PMID： 6276443      PMCID： PMC494595          DOI： 10.1136/jcp.34.12.1349

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  8 in total

1.  Enumeration of lymphocyte populations in whole peripheral blood with alkaline phosphatase-labelled reagents. A method for routine clinical use.

Authors:  M Druguet; M B Pepys
Journal:  Clin Exp Immunol       Date:  1977-07       Impact factor: 4.330

Review 2.  Immunoperoxidase technique in histopathology: applications, methods, and controls.

Authors:  E Heyderman
Journal:  J Clin Pathol       Date:  1979-10       Impact factor: 3.411

3.  Immunoperoxidase reactions in resin embedded sections.

Authors:  E Heyderman; P Monaghan
Journal:  Invest Cell Pathol       Date:  1979 Apr-Jun

4.  Letter: Destruction of endogenous peroxidase activity in order to locate cellular antigens by peroxidase-labeled antibodies.

Authors:  A Pitts; E E Williams
Journal:  J Histochem Cytochem       Date:  1974-01       Impact factor: 2.479

5.  Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol.

Authors:  J G Streefkerk
Journal:  J Histochem Cytochem       Date:  1972-10       Impact factor: 2.479

6.  Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.

Authors:  S Avrameas
Journal:  Immunochemistry       Date:  1969-01

7.  Immunoperoxidase technics in diagnostic pathology. Report of a workshop sponsored by the National Cancer Institute.

Authors:  R A DeLellis; L A Sternberger; R B Mann; P M Banks; P K Nakane
Journal:  Am J Clin Pathol       Date:  1979-05       Impact factor: 2.493

8.  Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents.

Authors:  D Y Mason; R Sammons
Journal:  J Clin Pathol       Date:  1978-05       Impact factor: 3.411
  8 in total
  5 in total

1.  Structural characterization of the mesangial cell type IV collagenase and enhanced expression in a model of immune complex-mediated glomerulonephritis.

Authors:  D H Lovett; R J Johnson; H P Marti; J Martin; M Davies; W G Couser
Journal:  Am J Pathol       Date:  1992-07       Impact factor: 4.307

Review 2.  Practical suggestions for successful immunoenzyme double-staining experiments.

Authors:  C M van der Loos; A E Becker; J J van den Oord
Journal:  Histochem J       Date:  1993-01

3.  Cellular localization of simian immunodeficiency virus in lymphoid tissues. I. Immunohistochemistry and electron microscopy.

Authors:  D J Ringler; M S Wyand; D G Walsh; J J MacKey; L V Chalifoux; M Popovic; A A Minassian; P K Sehgal; M D Daniel; R C Desrosiers
Journal:  Am J Pathol       Date:  1989-02       Impact factor: 4.307

4.  Immunohistochemical Detection of SARS-CoV-2 Antigens by Single and Multiple Immunohistochemistry.

Authors:  Silvia Lonardi; Mattia Bugatti; Arianna Valzelli; Fabio Facchetti
Journal:  Methods Mol Biol       Date:  2022

5.  Molecular characterization of a low-molecular-mass matrix metalloproteinase secreted by glomerular mesangial cells as PUMP-1.

Authors:  H P Marti; L McNeil; G Thomas; M Davies; D H Lovett
Journal:  Biochem J       Date:  1992-08-01       Impact factor: 3.857
  5 in total

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