Literature DB >> 35554913

Immunohistochemical Detection of SARS-CoV-2 Antigens by Single and Multiple Immunohistochemistry.

Silvia Lonardi1, Mattia Bugatti2, Arianna Valzelli1, Fabio Facchetti3.   

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can be demonstrated in tissue sections by immunohistochemistry (IHC), which has the power to localize in bright field specific antigens in cells and tissues. The use of double or triple immunostains is capable of highlighting which cells are infected and/or the relationship of infected cell with other cells and tissue structures. In addition, immunoenzymatic multi-staining permits the simultaneous identification, localization, and enumeration of different cellular epitopes. Moreover, this method improves analytical precision, decreasing the time required for morphometric quantification, maximizing the information obtained from a single slide of paraffin-embedded tissue.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Antibodies; Immunohistochemistry Double/Triple immunohistochemistry; SARS-CoV-2

Mesh：

Substances：

Year:  2022        PMID： 35554913     DOI： 10.1007/978-1-0716-2111-0_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  31 in total

Review 1.  Immunohistochemistry in the diagnosis of cutaneous bacterial infections.

Authors:  Ana María Molina-Ruiz; Lorenzo Cerroni; Heinz Kutzner; Luis Requena
Journal:  Am J Dermatopathol       Date:  2015-03       Impact factor: 1.533

Review 2.  Immunohistochemistry in the diagnosis of cutaneous viral infections- part II: cutaneous viral infections by parvoviruses, poxviruses, paramyxoviridae, picornaviridae, retroviruses and filoviruses.

Authors:  Ana M Molina-Ruiz; Carlos Santonja; Arno Rütten; Lorenzo Cerroni; Heinz Kutzner; Luis Requena
Journal:  Am J Dermatopathol       Date:  2015-02       Impact factor: 1.533

Review 3.  Immunohistochemistry in the diagnosis of cutaneous viral infections--part I. Cutaneous viral infections by herpesviruses and papillomaviruses.

Authors:  Ana M Molina-Ruiz; Carlos Santonja; Arno Rütten; Lorenzo Cerroni; Heinz Kutzner; Luis Requena
Journal:  Am J Dermatopathol       Date:  2015-01       Impact factor: 1.533

4.  Detection of SARS-CoV-2 in formalin-fixed paraffin-embedded tissue sections using commercially available reagents.

Authors:  Alejandro Best Rocha; Edana Stroberg; Lisa M Barton; Eric J Duval; Sanjay Mukhopadhyay; Nicole Yarid; Tiffany Caza; Jon D Wilson; Daniel J Kenan; Michael Kuperman; Shree G Sharma; Christopher P Larsen
Journal:  Lab Invest       Date:  2020-07-09       Impact factor: 5.662

5.  Insights into pathogenesis of fatal COVID-19 pneumonia from histopathology with immunohistochemical and viral RNA studies.

Authors:  Jennifer L Sauter; Marina K Baine; Kelly J Butnor; Darren J Buonocore; Jason C Chang; Achim A Jungbluth; Matthias J Szabolcs; Sejal Morjaria; Sharon L Mount; Natasha Rekhtman; Elena Selbs; Zong-Mei Sheng; Yongli Xiao; David E Kleiner; Stefania Pittaluga; Jeffery K Taubenberger; Amy V Rapkiewicz; William D Travis
Journal:  Histopathology       Date:  2020-10-16       Impact factor: 7.778

6.  Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques.

Authors:  Mariano Carossino; Hon S Ip; Juergen A Richt; Kendra Shultz; Kimberly Harper; Alan T Loynachan; Fabio Del Piero; Udeni B R Balasuriya
Journal:  Arch Virol       Date:  2020-08-05       Impact factor: 2.574

7.  Pathology and Pathogenesis of SARS-CoV-2 Associated with Fatal Coronavirus Disease, United States.

Authors:  Roosecelis B Martines; Jana M Ritter; Eduard Matkovic; Joy Gary; Brigid C Bollweg; Hannah Bullock; Cynthia S Goldsmith; Luciana Silva-Flannery; Josilene N Seixas; Sarah Reagan-Steiner; Timothy Uyeki; Amy Denison; Julu Bhatnagar; Wun-Ju Shieh; Sherif R Zaki
Journal:  Emerg Infect Dis       Date:  2020-05-21       Impact factor: 6.883

8.  SARS-CoV-2 endothelial infection causes COVID-19 chilblains: histopathological, immunohistochemical and ultrastructural study of seven paediatric cases.

Authors:  I Colmenero; C Santonja; M Alonso-Riaño; L Noguera-Morel; A Hernández-Martín; D Andina; T Wiesner; J L Rodríguez-Peralto; L Requena; A Torrelo
Journal:  Br J Dermatol       Date:  2020-08-05       Impact factor: 11.113

9.  Unspecific post-mortem findings despite multiorgan viral spread in COVID-19 patients.

Authors:  Myriam Remmelink; Ricardo De Mendonça; Nicky D'Haene; Sarah De Clercq; Camille Verocq; Laetitia Lebrun; Philomène Lavis; Marie-Lucie Racu; Anne-Laure Trépant; Calliope Maris; Sandrine Rorive; Jean-Christophe Goffard; Olivier De Witte; Lorenzo Peluso; Jean-Louis Vincent; Christine Decaestecker; Fabio Silvio Taccone; Isabelle Salmon
Journal:  Crit Care       Date:  2020-08-12       Impact factor: 9.097

10.  SARS-CoV-2 can infect the placenta and is not associated with specific placental histopathology: a series of 19 placentas from COVID-19-positive mothers.

Authors:  Jonathon L Hecht; Bradley Quade; Vikram Deshpande; Mari Mino-Kenudson; David T Ting; Niyati Desai; Beata Dygulska; Taryn Heyman; Carolyn Salafia; Dejun Shen; Sara V Bates; Drucilla J Roberts
Journal:  Mod Pathol       Date:  2020-08-02       Impact factor: 8.209
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