Literature DB >> 6275786

Virus inhibition assay for measurement of acyclovir levels in human plasma and urine.

D F Moore, S C Taylor, Y J Bryson.   

Abstract

A simple microplate virus inhibition assay which measures the levels of acyclovir in plasma and urine samples from patients was developed. The assay is based upon the inhibition of the cytopathic effect of herpes simplex virus type 1 on human fibroblast cells. The extent of inhibition of virus cytopathic effect, caused by dilutions of samples from patients, allowed determination of acyclovir concentrations to be made. The assay, which measured biological activity, could detect acyclovir levels of greater than or equal to 1.0 microM. Peak and valley levels measured in plasma samples from two patients were comparable to values obtained by radioimmunoassay. The assay is simple, rapid, and quantitative, and it can be adapted to routine use for a large number of specimens.

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Year:  1981        PMID： 6275786      PMCID： PMC181799          DOI： 10.1128/AAC.20.6.787

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  11 in total

1.  Thymidine kinase from herpes simplex virus phosphorylates the new antiviral compound, 9-(2-hydroxyethoxymethyl)guanine.

Authors:  J A Fyfe; P M Keller; P A Furman; R L Miller; G B Elion
Journal:  J Biol Chem       Date:  1978-12-25       Impact factor: 5.157

2.  Selectivity of action of an antiherpetic agent, 9-(2-hydroxyethoxymethyl) guanine.

Authors:  G B Elion; P A Furman; J A Fyfe; P de Miranda; L Beauchamp; H J Schaeffer
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

3.  9-(2-hydroxyethoxymethyl) guanine activity against viruses of the herpes group.

Authors:  H J Schaeffer; L Beauchamp; P de Miranda; G B Elion; D J Bauer; P Collins
Journal:  Nature       Date:  1978-04-13       Impact factor: 49.962

4.  Bioassay system for determining ribavirin levels in human serum and urine.

Authors:  D F Smee; R W Sidwell; B B Barnett; R S Spendlove
Journal:  Chemotherapy       Date:  1981       Impact factor: 2.544

5.  Growth inhibition by acycloguanosine of herpesviruses isolated from human infections.

Authors:  C S Crumpacker; L E Schnipper; J A Zaia; M J Levin
Journal:  Antimicrob Agents Chemother       Date:  1979-05       Impact factor: 5.191

6.  The activity in vitro against herpes virus of 9-(2-hydroxyethoxymethyl)guanine (acycloguanosine), a new antiviral agent.

Authors:  P Collins; D J Bauer
Journal:  J Antimicrob Chemother       Date:  1979-07       Impact factor: 5.790

7.  Simple sensitive microbioassay for adenine arabinoside and hypoxanthine arabinoside in human plasma.

Authors:  Y J Bryson; L Sweetman; J D Connor
Journal:  Antimicrob Agents Chemother       Date:  1978-12       Impact factor: 5.191

8.  A sensitive radioimmunoassay for the antiviral agent BW248U [9-(2-hydroxyethoxymethyl)guanine].

Authors:  R P Quinn; P de Miranda; L Gerald; S S Good
Journal:  Anal Biochem       Date:  1979-10-01       Impact factor: 3.365

9.  Efficacy of acycloguanosine (Wellcome 248U) against herpes-simplex corneal ulcers.

Authors:  B R Jones; D J Coster; P N Fison; G M Thompson; L M Cobo; M G Falcon
Journal:  Lancet       Date:  1979-02-03       Impact factor: 79.321

10.  Effect of acycloguanosine treatment of acute and latent herpes simplex infections in mice.

Authors:  H J Field; S E Bell; G B Elion; A A Nash; P Wildy
Journal:  Antimicrob Agents Chemother       Date:  1979-04       Impact factor: 5.191
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  2 in total

Review 1.  Clinical pharmacokinetics of acyclovir.

Authors:  O L Laskin
Journal:  Clin Pharmacokinet       Date:  1983 May-Jun       Impact factor: 6.447

Review 2.  Acyclovir. A review of its pharmacodynamic properties and therapeutic efficacy.

Authors:  D M Richards; A A Carmine; R N Brogden; R C Heel; T M Speight; G S Avery
Journal:  Drugs       Date:  1983-11       Impact factor: 9.546
  2 in total

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