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Literature DB >> 6275390

Transposon-specified site-specific recombination.

P Kitts, L Symington, M Burke, R Reed, D Sherratt.

Abstract

Cointegrate DNA molecules containing two copies of a transposable element appear to be intermediates in the transposition process. These structures are resolved by site-specific recombination to yield the normal end products of transposition. The transposable element gamma delta (Tn1000) synthesizes a product interchangeable with the Tn1/3tnpR protein in promoting Tn1/3 site-specific recombination. These data support the hypothesis that cointegrates containing directly repeated copies of Tn1/3 are obligatory intermediates in interreplicon transposition of Tn1/3. In addition, we show here that the reaction is independent of the element-encoded tnpA gene product. Tn501, which specifies mercury resistance, also produces cointegrates as intermediates in interreplicon transposition. The appearance of Tn501-specified recombination activity that can act on these cointegrates requires growth of cells in the presence of Hg2+.

Entities: Chemical

Mesh：

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Year: 1982 PMID： 6275390 PMCID： PMC345658 DOI： 10.1073/pnas.79.1.46

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

21 in total

3. A facile and reversible method to decrease the copy number of the ColE1-related cloning vectors commonly used in Escherichia coli.

Authors: M F Henry; J E Cronan
Journal: J Bacteriol Date: 1989-10 Impact factor: 3.490

10. Plasmid-borne cadmium resistance genes in Listeria monocytogenes are present on Tn5422, a novel transposon closely related to Tn917.

Authors: M Lebrun; A Audurier; P Cossart
Journal: J Bacteriol Date: 1994-05 Impact factor: 3.490

Transposon-specified site-specific recombination.

1. Chromosome transfer and Hfr formation by F in rec+ and recA strains of Escherichia coli K12.

2. Identification and characterization of a self-regulated repressor of translocation of the Tn3 element.

3. In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites.

4. The control region of the F sex factor DNA transfer cistrons: restriction mapping and DNA cloning.

5. Analysis of sequences transposed by complementation of two classes of transposition-deficient mutants of Tn3.

6. Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance element.

7. Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements.

8. The gamma delta sequence of F is an insertion sequence.

9. The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1.

10. Mercury resistance in a plasmid-bearing strain of Escherichia coli.

Review 1. Movable genetic elements and antibiotic resistance in enterococci.

2. Up-promoter mutations in the positively-regulated mer promoter of Tn501.

3. A facile and reversible method to decrease the copy number of the ColE1-related cloning vectors commonly used in Escherichia coli.

4. Nucleotide sequences required for Tn3 transposition immunity.

5. Transposition of Tn1000: in vivo properties.

6. Genetic analysis of Tn7 transposition.

7. Tn1721-encoded resolvase: structure of the tnpR gene and its in vitro functions.

8. Tn501 insertion mutagenesis in Pseudomonas aeruginosa PAO.

Review 9. Plasmid-determined resistance to antimicrobial drugs and toxic metal ions in bacteria.

10. Plasmid-borne cadmium resistance genes in Listeria monocytogenes are present on Tn5422, a novel transposon closely related to Tn917.