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Literature DB >> 2993811

Tn1721-encoded resolvase: structure of the tnpR gene and its in vitro functions.

Abstract

A 760 base pair nucleotide sequence of transposon Tn1721 containing the resolvase (tnpR) gene has been determined. A 186 triplet open reading frame was assigned to tnpR, and the allocation of -35 and -10 promoter boxes was supported by mapping transcription initiation at 70 base pairs upstream of tnpR. Expression of tnpR under tac promoter control generated sufficient resolvase protein for enzyme purification and for in vitro studies. Purified Tn1721 resolvase requires supercoiling and two directly oriented resolution (res) sites. The enzyme resolves cointegrate substrates containing repeat copies of Tn1721 res, of Tn21 res, of Tn21 res/Tn1721 res, but not of Tn3 res.

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Year: 1985 PMID： 2993811 DOI： 10.1007/bf00383332

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

23 in total

1. Uniform nomenclature for bacterial plasmids: a proposal.

Authors: R P Novick; R C Clowes; S N Cohen; R Curtiss; N Datta; S Falkow
Journal: Bacteriol Rev Date: 1976-03

2. Transposon-specified site-specific recombination.

Authors: P Kitts; L Symington; M Burke; R Reed; D Sherratt
Journal: Proc Natl Acad Sci U S A Date: 1982-01 Impact factor: 11.205

3. Transposon-mediated site-specific recombination: a defined in vitro system.

Authors: R R Reed
Journal: Cell Date: 1981-09 Impact factor: 41.582

4. Cleavage of the site-specific recombination protein gamma delta resolvase: the smaller of two fragments binds DNA specifically.

Authors: S S Abdel-Meguid; N D Grindley; N S Templeton; T A Steitz
Journal: Proc Natl Acad Sci U S A Date: 1984-04 Impact factor: 11.205

5. Resolution of a hybrid cointegrate between transposons Tn501 and Tn1721 defines the recombination site.

Authors: P Rogowsky; R Schmitt
Journal: Mol Gen Genet Date: 1984

6. Site-specific relaxation and recombination by the Tn3 resolvase: recognition of the DNA path between oriented res sites.

Authors: M A Krasnow; N R Cozzarelli
Journal: Cell Date: 1983-04 Impact factor: 41.582

5. A resolvase-like protein is required for the site-specific integration of the temperate lactococcal bacteriophage TP901-1.

Authors: B Christiansen; L Brøndsted; F K Vogensen; K Hammer
Journal: J Bacteriol Date: 1996-09 Impact factor: 3.490

6. Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli.

Authors: C Aslanidis; K Schmid; R Schmitt
Journal: J Bacteriol Date: 1989-12 Impact factor: 3.490

Tn1721-encoded resolvase: structure of the tnpR gene and its in vitro functions.

1. Uniform nomenclature for bacterial plasmids: a proposal.

2. Transposon-specified site-specific recombination.

3. Transposon-mediated site-specific recombination: a defined in vitro system.

4. Cleavage of the site-specific recombination protein gamma delta resolvase: the smaller of two fragments binds DNA specifically.

5. Resolution of a hybrid cointegrate between transposons Tn501 and Tn1721 defines the recombination site.

6. Site-specific relaxation and recombination by the Tn3 resolvase: recognition of the DNA path between oriented res sites.

7. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.

8. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.

9. Evidence for two functional gal promoters in intact Escherichia coli cells.

10. The resolvase protein from the transposon Tn21.

1. Specificity of DNA recognition in the nucleoprotein complex for site-specific recombination by Tn21 resolvase.

2. Site-specific recombination and topoisomerization by Tn21 resolvase: role of metal ions.

3. Partitioning of broad-host-range plasmid RP4 is a complex system involving site-specific recombination.

4. Toluene transposons Tn4651 and Tn4653 are class II transposons.

5. A resolvase-like protein is required for the site-specific integration of the temperate lactococcal bacteriophage TP901-1.

6. Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli.

7. The resolvase protein from the transposon Tn21.

8. Genetic analysis of a transposon carrying toluene degrading genes on a TOL plasmid pWW0.

9. Definition of three resolvase binding sites at the res loci of Tn21 and Tn1721.