Literature DB >> 6275386

Purification of chicken intestinal receptor for 1 alpha, 25-dihydroxyvitamin D3 to apparent homogeneity.

R U Simpson, H F DeLuca.   

Abstract

The chicken intestinal 1 alpha, 25-dihydroxyvitamin D3 receptor-like protein has been purified to apparent homogeneity as determined by sodium dodecyl sulfate gel electrophoresis. The techniques employed for the purification include selective precipitation of the receptor by Polymin P (polyethyleneimine) and (NH4)2SO4 and batch adsorption to and selective elution from hydroxylapatite, followed by gel exclusion and DEAE-cellulose chromatography. Finally, the labeled receptor was eluted at a pH of approximately 6.0 on a chromatofocusing column. The protein was purified 6100-fold and the receptor was obtained in 9% yield.

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Year:  1982        PMID： 6275386      PMCID： PMC345652          DOI： 10.1073/pnas.79.1.16

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  Purification of estrogen receptors. I.

Authors:  G A Puca; E Nola; V Sica; F Bresciani
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

2.  Equilibrium binding of estradiol by uterine cell suspensions and whole uteri in vitro.

Authors:  D Williams; J Gorski
Journal:  Biochemistry       Date:  1974-12-31       Impact factor: 3.162

3.  The association of a metabolite of vitamin D3 with intestinal mucosa chromatin in vivo.

Authors:  M R Haussler; J F Myrtle; A W Norman
Journal:  J Biol Chem       Date:  1968-08-10       Impact factor: 5.157

4.  An equilibrium and kinetic study of 1,25-dihydroxyvitamin D3 binding to chicken intestinal cytosol employing high specific activity 1,25-dehydroxy[3H-26, 27] vitamin D3.

Authors:  W S Mellon; H F DeLuca
Journal:  Arch Biochem Biophys       Date:  1979-10-01       Impact factor: 4.013

5.  Unoccupied 1,25-dihydroxyvitamin D3 receptors. Nuclear/cytosol ratio depends on ionic strength.

Authors:  M R Walters; W Hunziker; A W Norman
Journal:  J Biol Chem       Date:  1980-07-25       Impact factor: 5.157

6.  Partial purification of the chick intestinal receptor for 1,25-dihydroxy vitamin D by ion exchange and blue dextran-Sepharose chromatography.

Authors:  T A McCain; M R Haussler; D Okrent; M R Hughes
Journal:  FEBS Lett       Date:  1978-02-01       Impact factor: 4.124

7.  Aggregation properties of the 1,25-dihydroxyvitamin D3 receptor from chick intestinal cytosol.

Authors:  R T Franceschi; H F DeLuca
Journal:  J Biol Chem       Date:  1979-11-25       Impact factor: 5.157

8.  Purification of chicken intestinal receptor for 1,25-dihydroxyvitamin D.

Authors:  J W Pike; M R Haussler
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

9.  Characterization of a specific, high affinity binding macromolecule for 1 alpha, 25-dihydroxyvitamin D3 in cultured chick kidney cells.

Authors:  R U Simpson; R T Franceschi; H F DeLuca
Journal:  J Biol Chem       Date:  1980-11-10       Impact factor: 5.157

10.  Actinomycin D Inhibition of Vitamin D Action.

Authors:  J E Zull; E Czarnowska-Misztal; H F Deluca
Journal:  Science       Date:  1965-07-09       Impact factor: 47.728
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  8 in total

1.  Structure and regulation of the rat 1,25-dihydroxyvitamin D3 receptor.

Authors:  J K Burmester; R J Wiese; N Maeda; H F DeLuca
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

Review 2.  Vitamin D-endocrine system.

Authors:  N H Bell
Journal:  J Clin Invest       Date:  1985-07       Impact factor: 14.808

3.  Partial amino acid sequence of porcine 1,25-dihydroxyvitamin D3 receptor isolated by immunoaffinity chromatography.

Authors:  T A Brown; J M Prahl; H F DeLuca
Journal:  Proc Natl Acad Sci U S A       Date:  1988-04       Impact factor: 11.205

4.  Subcellular distribution of DNA-binding and non-DNA-binding 1,25-dihydroxyvitamin D receptors in chicken intestine.

Authors:  M Nakada; R U Simpson; H F DeLuca
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

5.  Efficient stable isotope labeling and purification of vitamin D receptor from inclusion bodies.

Authors:  Jinge Zhu; Hongyu Rao; Marco Tonelli; William M Westler; Kiran K Singarapu; John L Markley; Hector F DeLuca; Fariba M Assadi-Porter
Journal:  Protein Expr Purif       Date:  2012-06-29       Impact factor: 1.650

6.  Development of hybridomas secreting monoclonal antibodies to the chicken intestinal 1 alpha,25-dihydroxyvitamin D3 receptor.

Authors:  J W Pike; C A Donaldson; S L Marion; M R Haussler
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

7.  Specific binding of 1,25-dihydroxycholecalciferol in human medullary thyroid carcinoma.

Authors:  H C Freake; I MacIntyre
Journal:  Biochem J       Date:  1982-07-15       Impact factor: 3.857

8.  Quantitation of endogenously occupied and unoccupied binding sites for 1,25-dihydroxyvitamin D3 in rat intestine.

Authors:  E R Massaro; R U Simpson; H F DeLuca
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205
  8 in total

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