Literature DB >> 6271899

The detection of DNA tumour virus-specific RNA sequences in abnormal human cervical biopsies by in situ hybridization.

N J Maitland, J H Kinross, A Busuttil, S M Ludgate, G E Smart, K W Jones.   

Abstract

We have used the technique of in situ nucleic acid hybridization and autoradiography of thin frozen sections of human tissue to search for virus RNA sequences in human cervical tumours. Of cervical biopsies with abnormal cytology, 67% bound herpes simplex virus type 2 (HSV2) 3H-labelled DNA probes and 39% bound adenovirus type 2 (Ad2) 3H-labelled DNA probes, whereas control experiments with phage lambda 3H-labelled DNA probes, under the same conditions, bound to only 7% of cases. In contrast, normal cervical biopsies bound the three probes in only 23%, 17% and 8% of cases respectively.

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Year:  1981        PMID： 6271899     DOI： 10.1099/0022-1317-55-1-123

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  14 in total

1.  Histological aspects of in situ hybridization. Detection of poly(A) nucleotide sequences in mouse liver sections as a model system.

Authors:  A R Jilbert; C J Burrell; E J Gowans; R Rowland
Journal:  Histochemistry       Date:  1986

2.  The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures.

Authors:  L Singh; K W Jones
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

3.  Genital herpes: hype or hope?

Authors:  M W Alder; A Mindel
Journal:  Br Med J (Clin Res Ed)       Date:  1983-06-04

4.  RNA from an immediate early region of the type 1 herpes simplex virus genome is present in the trigeminal ganglia of latently infected mice.

Authors:  A M Deatly; J G Spivack; E Lavi; N W Fraser
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

5.  Failure to detect viral genomic sequences of three viruses (herpes simplex, simian virus 40 and adenovirus) in human and rat brain tumors.

Authors:  C Chauvin; M Suh; C Remy; A L Benabid
Journal:  Ital J Neurol Sci       Date:  1990-08

6.  Induction of cervical neoplasia in the mouse by herpes simplex virus type 2 DNA.

Authors:  D D Anthony; W B Wentz; J W Reagan; A D Heggie
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205

7.  Restriction endonuclease analysis of DNA from genital isolates of herpes simplex virus type 2.

Authors:  N J Maitland; I W Smith; J F Peutherer; D H Robertson; K W Jones
Journal:  Infect Immun       Date:  1982-12       Impact factor: 3.441

8.  Herpesvirus-specific RNA and protein in carcinoma of the uterine cervix.

Authors:  J K McDougall; C P Crum; C M Fenoglio; L C Goldstein; D A Galloway
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205

9.  Comparison of prevalence of human papillomavirus antigen in biopsies from women with cervical intraepithelial neoplasia.

Authors:  A Singer; J Wilters; P Walker; D Jenkins; G Slavin; H Cowdell; A To; O Husain
Journal:  J Clin Pathol       Date:  1985-08       Impact factor: 3.411

10.  The pattern of expression of chick delta-crystallin genes in lens differentiation and in trans-differentiating cultured tissues.

Authors:  D J Bower; L H Errington; B J Pollock; S Morris; R M Clayton
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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