Literature DB >> 6269060

Cloning of multiple copies of immunoglobulin variable kappa genes in cosmid vectors.

R Cattaneo, J Gorski, B Mach.   

Abstract

The possibility of cloning large segments of DNA in cosmid vectors offers distinct advantages, in particular for the study of multigene families. Large size fragments of mouse embryo DNA were successfully cloned in the cosmid pHC 79. Twelve recombinants hybridizing specifically to an immunoglobulin kappa chain variable region probe were identified. In 9 of these recombinants, the size of the insert ranges from 30 to 43 kilobases. Factors affecting the cloning efficiency of a complex mammalian genome in cosmids were studied. The stability of these recombinant cosmids and the preparation of recombinant cosmid DNA are also discussed.

Entities:  

Mesh：

Substances：

Year:  1981        PMID： 6269060      PMCID： PMC326892          DOI： 10.1093/nar/9.12.2777

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.

Authors:  J B Hansen; R H Olsen
Journal:  J Bacteriol       Date:  1978-07       Impact factor: 3.490

4.  Packaging recombinant DNA molecules into bacteriophage particles in vitro.

Authors:  B Hohn; K Murray
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205

5.  Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors:  W D Benton; R W Davis
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

6.  Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.

Authors:  F R Blattner; B G Williams; A E Blechl; K Denniston-Thompson; H E Faber; L Furlong; D J Grunwald; D O Kiefer; D D Moore; J W Schumm; E L Sheldon; O Smithies
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

7.  Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions.

Authors:  N Hozumi; S Tonegawa
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

8.  Isolation of high-molecular-weight DNA from mammalian cells.

Authors:  M Gross-Bellard; P Oudet; P Chambon
Journal:  Eur J Biochem       Date:  1973-07-02

9.  The rabbit beta-globin gene contains a large large insert in the coding sequence.

Authors:  A J Jeffreys; R A Flavell
Journal:  Cell       Date:  1977-12       Impact factor: 41.582

10.  Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.

Authors:  L Katz; D T Kingsbury; D R Helinski
Journal:  J Bacteriol       Date:  1973-05       Impact factor: 3.490
View more
  1 in total

1.  Patents and literature.

Authors:  A Rembaum; R P Rohrbach; S Klose; H G Batz; M Stoltz; A Hagen; G Weimann; S G Wildman; P Kwanyuen; G Franzmann; H L Wulsmann; T Nakanishi; Y Shigemasa; R Monsheimer; E Pfleiderer; T Yoshioka; K Teramoto; M Shimamura; C K Lee; M Tamuri; M Kanno; Y Ishi; I Kaetsu; M Kumakura; M Yoshida; G A Strobel; D E Johnson; V R Srinivasan; Y C Choi; H Fukuda; T Shiotani; W Okada; R A Messing; R A Oppermann; L B Simpson; M M Takeguchi; M Bigazzi; T B Higerd; J M Goust; W R Tolbert; M J Kuo; J Feder; R N Patel; C T Hou; A I Laskin; H Yukawa; K Osumi; Y Takayama; A Fujiwara; S Masuda; F Takaku; K Ogasa; M Kuboyama; M Saito; N Yanai; M Nishida; O Tosaka; E Ono; M Ishihara; H Morioka; K Takinami; A Taoka; S Uchida; J Puls; M Sinner; H H Dietrichs; D T Wicklow; R W Detroy; R E Heady; H Yukawa; K Osumi; T Nara; Y Takayama; E Knight; G T Tsao; M R Ladisch; C M Ladisch; T A Hsu; H J Friesen; S Pestka; J J Manis; V G Debabov; J I Kozlov; N I Zhdanova; E M Khurges; N K Yankovsky; M R Rozinov; R S Shakulov; B A Rebentish; V A Livshits; M M Gusyatiner; S V Mashko; V N Moshentseva; L F Kozyreva; R A Arsatiants; H M Goodman; J Shine; P Horst; D V Goeddel; H L Heyneker
Journal:  Appl Biochem Biotechnol       Date:  1981-12       Impact factor: 2.926
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.