Literature DB >> 6268936

Cosmid cloning and transposon mutagenesis in Salmonella typhimurium using phage lambda vehicles.

E T Palva, P Liljeström, S Harayama.   

Abstract

We have constructed a strain of Salmonella typhimurium which contains the malB region from Escherichia coli and carries the bacteriophage lambda receptor protein in its outer membrane. Phage lambda adsorbs to this strain but cannot grow, thus providing a very useful system for transposon mutagenesis of S. typhimurium using lambda vehicles carrying transposons. This system can also be used for cosmid cloning.

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Year:  1981        PMID： 6268936     DOI： 10.1007/bf00268420

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  26 in total

1.  Escherichia coli plasmids packageable in vitro in lambda bacteriophage particles.

Authors:  J Collins
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

2.  Structure of the malB region in Escherichia coli K12. I. Genetic map of the malK-lamB operon.

Authors:  O Raibaud; M Roa; C Braun-Breton; M Schwartz
Journal:  Mol Gen Genet       Date:  1979-07-24

3.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors:  D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

4.  Translocatable elements in procaryotes.

Authors:  N Kleckner
Journal:  Cell       Date:  1977-05       Impact factor: 41.582

5.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors:  G Fairbanks; T L Steck; D F Wallach
Journal:  Biochemistry       Date:  1971-06-22       Impact factor: 3.162

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Occurrence of the bacteriophage lambda receptor in some enterobacteriaceae.

Authors:  M Schwartz; L Le Minor
Journal:  J Virol       Date:  1975-04       Impact factor: 5.103

8.  E. coli K-12 pel mutants, which block phage lambda DNA injection, coincide with ptsM, which determines a component of a sugar transport system.

Authors:  J Elliott; W Arber
Journal:  Mol Gen Genet       Date:  1978-04-25

9.  Plasmids useable as gene-cloning vectors in an in vitro packaging by coliphage lambda: "cosmids".

Authors:  J Collins; H J Brüning
Journal:  Gene       Date:  1978-10       Impact factor: 3.688

10.  DNA restriction and modification systems in Salmonella. I. SA and SB, two Salmonella typhimurium systems determined by genes with a chromosomal location comparable to that of the Escherichia coli hsd genes.

Authors:  C Colson; A Van Pel
Journal:  Mol Gen Genet       Date:  1974-04-03
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  26 in total

1.  Use of lambda vehicles to isolate ompC-lacZ gene fusions in Salmonella typhimurium LT2.

Authors:  A Harkki; H Karkku; E T Palva
Journal:  Mol Gen Genet       Date:  1987-10

2.  Mutations in rpoA affect expression of anaerobically regulated genes in Salmonella typhimurium.

Authors:  M J Lombardo; D Bagga; C G Miller
Journal:  J Bacteriol       Date:  1991-12       Impact factor: 3.490

3.  Affinity Immobilization of Escherichia coli: Catalysis by Intact and Permeable Cells Bound to Starch.

Authors:  T Ferenci
Journal:  Appl Environ Microbiol       Date:  1983-02       Impact factor: 4.792

4.  Isolation and characterization of mutants with deletions in dnaQ, the gene for the editing subunit of DNA polymerase III in Salmonella typhimurium.

Authors:  E D Lancy; M R Lifsics; D G Kehres; R Maurer
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

5.  Bacteriophage lambda-mediated transposon mutagenesis of phytopathogenic and epiphytic Erwinia species is strain dependent.

Authors:  F M Ellard; A Cabello; G P Salmond
Journal:  Mol Gen Genet       Date:  1989-09

6.  New Salmonella typhimurium mutants with altered outer membrane permeability.

Authors:  S Sukupolvi; M Vaara; I M Helander; P Viljanen; P H Mäkelä
Journal:  J Bacteriol       Date:  1984-08       Impact factor: 3.490

7.  Two genetically distinct pathways for transcriptional regulation of anaerobic gene expression in Salmonella typhimurium.

Authors:  D J Jamieson; C F Higgins
Journal:  J Bacteriol       Date:  1986-10       Impact factor: 3.490

8.  Two alanine racemase genes in Salmonella typhimurium that differ in structure and function.

Authors:  S A Wasserman; C T Walsh; D Botstein
Journal:  J Bacteriol       Date:  1983-03       Impact factor: 3.490

9.  Cloning of the regulatory locus ompB of Salmonella typhimurium LT-2. I. Isolation of the ompR gene and identification of its gene product.

Authors:  P Liljeström; P L Määttänen; E T Palva
Journal:  Mol Gen Genet       Date:  1982

10.  Application of phage lambda technology to Salmonella typhimurium. Construction of a lambda-sensitive Salmonella strain.

Authors:  A Harkki; E T Palva
Journal:  Mol Gen Genet       Date:  1984
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