Cessation of reentry of simian virus 40 DNA into replication and its simultaneous appearance in nucleoprotein complexes of the maturation pathway.

Newly synthesized SV40 DNA is used as a template for further DNA synthesis (reenters replication) or as a substrate in the assembly of virions (maturation pathway). The time courses of reentry into replication and progression along the maturation pathway were both determined on identical samples. DNA, synthesized during a 20-min pulse, reentered replication over a period of several hours and then was removed from the pool of molecules available for replication. The cessation of reentry coincided with the maturation of this DNA from the chromatin form to previrion and virion forms. More reentry and less maturation was observed at 24 h postinfection than at 42 h postinfection. The data are consistent with the hypothesis that the factor(s) responsible for cessation of reentry is also responsible for initiation of the maturation pathway.