Literature DB >> 6268017

Detection of HBsAg in a clone derived from the PLC/PRF/5 human hepatoma cell line.

Y M Wen, J A Copeland, G F Mann, C R Howard, A J Zuckerman.   

Abstract

A total of 28 clones were established from the PLC/PRF/5 hepatoma cell line by a plating procedure. All clones were found to secrete HBsAg into the supernatant culture fluids. Of these, one clone (No. 23) free of detectable mycoplasma contamination and showing smooth epithelial morphology was selected for further study. Maximum accumulation of HBsAg occurred 9 days after sub-culture and intracellular antigen was detected by indirect immunofluorescence both in the cytoplasm and at the plasma membrane. Granules and perinuclear staining reactions were also observed in clone 23 cells and these findings are compared to the previously published properties of the parental PLC/PRF/5 cell line.

Entities:  

Mesh：

Substances：

Year:  1981        PMID： 6268017     DOI： 10.1007/bf01314568

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  9 in total

1.  Hepatitis B surface antigen produced by a human hepatoma cell line.

Authors:  J Skelly; J A Copeland; C R Howard; A J Zuckerman
Journal:  Nature       Date:  1979-12-06       Impact factor: 49.962

2.  Hepatitis B surface antigen production as a growth cycle-related terminal event in PLC/PRF/5 hepatoma cells.

Authors:  J A Copeland; J Skelly; G F Mann; C R Howard; A J Zuckerman
Journal:  J Med Virol       Date:  1980       Impact factor: 2.327

3.  Identification of integrated hepatitis B virus DNA and expression of viral RNA in an HBsAg-producing human hepatocellular carcinoma cell line.

Authors:  P R Chakraborty; N Ruiz-Opazo; D Shouval; D A Shafritz
Journal:  Nature       Date:  1980-07-31       Impact factor: 49.962

4.  Electron microscopy of HBsAg from human hepatoma cell line.

Authors: 
Journal:  Lancet       Date:  1977-10-01       Impact factor: 79.321

5.  Polypeptides of hepatitis B virus surface antigen produced by a hepatoma cell line.

Authors:  P L Marion; F H Salazar; J J Alexander; W S Robinson
Journal:  J Virol       Date:  1979-12       Impact factor: 5.103

6.  Integration of hepatitis B virus sequences and their expression in a human hepatoma cell.

Authors:  J C Edman; P Gray; P Valenzuela; L B Rall; W J Rutter
Journal:  Nature       Date:  1980-07-31       Impact factor: 49.962

7.  Nondetection of infectious hepatitis B virus in a human hepatoma cell line producing hepatitis B surface antigen.

Authors:  E Tabor; J A Copeland; G F Mann; C R Howard; J Skelly; P Snoy; A J Zuckerman; R J Gerety
Journal:  Intervirology       Date:  1981       Impact factor: 1.763

8.  Establishment of a continuously growing cell line from primary carcinoma of the liver.

Authors:  J J Alexander; E M Bey; E W Geddes; G Lecatsas
Journal:  S Afr Med J       Date:  1976-12-18

9.  Hepatitis B surface antigen produced by a human hepatoma cell line.

Authors:  G M MacNab; J J Alexander; G Lecatsas; E M Bey; J M Urbanowicz
Journal:  Br J Cancer       Date:  1976-11       Impact factor: 7.640
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.