Literature DB >> 6263223

The influence of divalent cations on the stability of human rotavirus.

J A Shirley, G M Beards, M E Thouless, T H Flewett.   

Abstract

The influence of divalent cations on the stability of human rotavirus was investigated using the indirect immunofluorescence (FA) technique in LLC-MK2 cells to titrate infectivity. Rotavirus infectivity was stabilized by calcium and strontium ions in solution but not by magnesium ions. Rotavirus isolates were found to be much less stable at 37 degrees C than at + 4 degrees C or 20 degrees. A study of virus morphology at intervals during the course of the experiment and treatment with the chelating agents EDTA and EGTA suggests that loss of infectivity coincides with the removal of the outer capsid layer and that calcium may be required to maintain virus integrity.

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Year:  1981        PMID： 6263223     DOI： 10.1007/bf01314596

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  10 in total

Review 1.  Review of calcium methodologies.

Authors:  B Zak; E Epstein; E S Baginski
Journal:  Ann Clin Lab Sci       Date:  1975 May-Jun       Impact factor: 1.256

2.  Separation and infectivity of two particle types of human rotavirus.

Authors:  M M Elias
Journal:  J Gen Virol       Date:  1977-10       Impact factor: 3.891

3.  Characterization of two particle types of calf rotavirus.

Authors:  J C Bridger; G N Woode
Journal:  J Gen Virol       Date:  1976-05       Impact factor: 3.891

4.  Ribonucleic acid polymerase activity associated with purified calf rotavirus.

Authors:  J Cohen
Journal:  J Gen Virol       Date:  1977-09       Impact factor: 3.891

5.  Relation between viruses from acute gastroenteritis of children and newborn calves.

Authors:  T H Flewett; A S Bryden; H Davies; G N Woode; J C Bridger; J M Derrick
Journal:  Lancet       Date:  1974-07-13       Impact factor: 79.321

6.  Activation of rotavirus RNA polymerase by calcium chelation.

Authors:  J Cohen; J Laporte; A Charpilienne; R Scherrer
Journal:  Arch Virol       Date:  1979       Impact factor: 2.574

7.  Diagnostic electron microscopy of faeces. II. Acute gastroenteritis associated with reovirus-like particles.

Authors:  T H Flewett; H Davies; A S Bryden; M J Robertson
Journal:  J Clin Pathol       Date:  1974-08       Impact factor: 3.411

8.  Rotavirus stability and inactivation.

Authors:  M K Estes; D Y Graham; E M Smith; C P Gerba
Journal:  J Gen Virol       Date:  1979-05       Impact factor: 3.891

9.  Diagnosis of rotavirus infection by cell culture.

Authors:  A S Bryden; H A Davies; M E Thouless; T H Flewitt
Journal:  J Med Microbiol       Date:  1977-02       Impact factor: 2.472

10.  Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization.

Authors:  M E Thouless; A S Bryden; T H Flewett; G N Woode; J C Bridger; D R Snodgrass; J A Herring
Journal:  Arch Virol       Date:  1977       Impact factor: 2.574
  10 in total
  15 in total

1.  Interaction of rotavirus particles with liposomes.

Authors:  P Nandi; A Charpilienne; J Cohen
Journal:  J Virol       Date:  1992-06       Impact factor: 5.103

2.  Infectivity and antigenicity reduction rates of human rotavirus strain Wa in fresh waters.

Authors:  O C Pancorbo; B G Evanshen; W F Campbell; S Lambert; S K Curtis; T W Woolley
Journal:  Appl Environ Microbiol       Date:  1987-08       Impact factor: 4.792

3.  Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3.

Authors:  R D Shaw; D L Stoner-Ma; M K Estes; H B Greenberg
Journal:  J Clin Microbiol       Date:  1985-08       Impact factor: 5.948

4.  Mechanism of inactivation of enteric viruses in fresh water.

Authors:  R L Ward; D R Knowlton; P E Winston
Journal:  Appl Environ Microbiol       Date:  1986-09       Impact factor: 4.792

5.  The nucleocapsid of the lipid-containing double-stranded RNA bacteriophage phi 6 contains a protein skeleton consisting of a single polypeptide species.

Authors:  V M Olkkonen; D H Bamford
Journal:  J Virol       Date:  1987-08       Impact factor: 5.103

6.  Occurrence of changes in human rotavirus serotypes with concurrent changes in genomic RNA electropherotypes.

Authors:  T Nakagomi; K Akatani; N Ikegami; N Katsushima; O Nakagomi
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948

7.  HT-29 cells: a new substrate for rotavirus growth.

Authors:  F Superti; A Tinari; L Baldassarri; G Donelli
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

8.  Comparison of six methods for detecting human rotavirus in stools.

Authors:  F Morinet; F Ferchal; R Colimon; Y Pérol
Journal:  Eur J Clin Microbiol       Date:  1984-04       Impact factor: 3.267

9.  Culturing, storage, and quantification of rotaviruses.

Authors:  Michelle Arnold; John T Patton; Sarah M McDonald
Journal:  Curr Protoc Microbiol       Date:  2009-11

10.  Expression of nonstructural rotavirus protein NSP4 mimics Ca2+ homeostasis changes induced by rotavirus infection in cultured cells.

Authors:  Yuleima Díaz; Maria Elena Chemello; Franshelle Peña; Olga Carolina Aristimuño; Jose Luis Zambrano; Hector Rojas; Fulvia Bartoli; Leiria Salazar; Serge Chwetzoff; Catherine Sapin; Germain Trugnan; Fabian Michelangeli; Marie Christine Ruiz
Journal:  J Virol       Date:  2008-09-10       Impact factor: 5.103
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