Literature DB >> 6262811

Transformation of mouse fibroblasts to methotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reductase.

K O'Hare, C Benoist, R Breathnach.   

Abstract

A recombinant plasmid has been constructed for the expression of inserted DNA sequences coding for polypeptide chains using the simian virus 40 early promoter and splicing and polyadenylylation signals from the rabbit beta-globin gene. The coding regions for two prokaryotic methotrexate-resistant dihydrofolate reductases were introduced into the expression vector. When mouse fibroblasts were exposed to these recombinant plasmids, it was possible to select methotrexate-resistant clones that had integrated the plasmids and produced a chimeric RNA coding for the prokaryotic enzyme.

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Year:  1981        PMID： 6262811      PMCID： PMC319164          DOI： 10.1073/pnas.78.3.1527

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  32 in total

1.  SV40 recombinants carrying rabbit beta-globin gene coding sequences.

Authors:  D H Hamer; K D Smith; S H Boyer; P Leder
Journal:  Cell       Date:  1979-07       Impact factor: 41.582

2.  SV40 recombinants carrying a functional RNA splice junction and polyadenylation site from the chromosomal mouse beta maj globin gene.

Authors:  D H Hamer; P Leder
Journal:  Cell       Date:  1979-07       Impact factor: 41.582

3.  Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.

Authors:  J C Alwine; D J Kemp; B A Parker; J Reiser; J Renart; G R Stark; G M Wahl
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

4.  The amino acid sequence of the trimethoprim-resistant dihydrofolate reductase specified in Escherichia coli by R-plasmid R67.

Authors:  D Stone; S L Smith
Journal:  J Biol Chem       Date:  1979-11-10       Impact factor: 5.157

5.  Structure and genomic organization of the mouse dihydrofolate reductase gene.

Authors:  J H Nunberg; R J Kaufman; A C Chang; S N Cohen; R T Schimke
Journal:  Cell       Date:  1980-02       Impact factor: 41.582

6.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  Protein expression in Escherichia coli minicells containing recombinant plasmids specifying trimethoprim-resistant dihydrofolate reductases.

Authors:  M E Fling; L P Elwell
Journal:  J Bacteriol       Date:  1980-02       Impact factor: 3.490

8.  Insertion of a new gene of viral origin into bone marrow cells of mice.

Authors:  K E Mercola; H D Stang; J Browne; W Salser; M J Cline
Journal:  Science       Date:  1980-05-30       Impact factor: 47.728

9.  Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels.

Authors:  N Rave; R Crkvenjakov; H Boedtker
Journal:  Nucleic Acids Res       Date:  1979-08-10       Impact factor: 16.971

10.  Corrected splicing of a chicken ovalbumin gene transcript in mouse L cells.

Authors:  R Breathnach; N Mantei; P Chambon
Journal:  Proc Natl Acad Sci U S A       Date:  1980-02       Impact factor: 11.205
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  42 in total

1.  FPL 64176 modification of Ca(V)1.2 L-type calcium channels: dissociation of effects on ionic current and gating current.

Authors:  Stefan I McDonough; Yasuo Mori; Bruce P Bean
Journal:  Biophys J       Date:  2004-10-22       Impact factor: 4.033

2.  pEF-BOS, a powerful mammalian expression vector.

Authors:  S Mizushima; S Nagata
Journal:  Nucleic Acids Res       Date:  1990-09-11       Impact factor: 16.971

3.  Expression of the plasmid-encoded type I dihydrofolate reductase gene in cultured mammalian cells: a novel selectable marker.

Authors:  C S Simonsen; M Walter; A D Levinson
Journal:  Nucleic Acids Res       Date:  1988-03-25       Impact factor: 16.971

4.  Mutational analysis of the human nucleotide excision repair gene ERCC1.

Authors:  A M Sijbers; P J van der Spek; H Odijk; J van den Berg; M van Duin; A Westerveld; N G Jaspers; D Bootsma; J H Hoeijmakers
Journal:  Nucleic Acids Res       Date:  1996-09-01       Impact factor: 16.971

5.  Constitutive production of human interferons by mouse cells with bovine papillomavirus as a vector.

Authors:  R Fukunaga; Y Sokawa; S Nagata
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205

6.  Osteoarthritis associated with mild chondrodysplasia in transgenic mice expressing alpha 1(IX) collagen chains with a central deletion.

Authors:  K Nakata; K Ono; J Miyazaki; B R Olsen; Y Muragaki; E Adachi; K Yamamura; T Kimura
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205

7.  Efficient expression of Escherichia coli galactokinase gene in mammalian cells.

Authors:  D Schümperli; B H Howard; M Rosenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1982-01       Impact factor: 11.205

8.  Construction of a mammalian transducing vector from the genome of Moloney murine leukemia virus.

Authors:  E Gilboa; M Kolbe; K Noonan; R Kucherlapati
Journal:  J Virol       Date:  1982-12       Impact factor: 5.103

9.  Use of recombinant DNA technology to program eukaryotic cells to synthesize rat proinsulin: a rapid expression assay for cloned genes.

Authors:  P T Lomedico
Journal:  Proc Natl Acad Sci U S A       Date:  1982-10       Impact factor: 11.205

10.  Mammalian cells transfected with the listeriolysin gene exhibit enhanced proliferation and focus formation.

Authors:  A Demuth; T Chakraborty; G Krohne; W Goebel
Journal:  Infect Immun       Date:  1994-11       Impact factor: 3.441
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