Literature DB >> 1903503

Molecular cloning and analysis of the chromosomal region 26A of Drosophila melanogaster.

D C Knipple1, T R Fuerst, R J MacIntyre.   

Abstract

Region 26A of the second chromosome of Drosophila melanogaster has been extensively characterized at the genetic level. We report here the cloning of virtually the entire 26A region via a bidirectional chromosome walk. Deletion and translocation breakpoints in the 26A interval have been localized at the molecular level by both chromosomal in situ hybridization and Southern analysis. The locations of the genetically defined loci in this chromosomal region have also been correlated with transcriptional units mapped onto the DNA of the proximal region of the chromosomal walk. The position of the alpha-glycerophosphate dehydrogenase (alpha-Gpdh) gene in 26A5-7 has been confirmed and a putative transcriptional unit for the beta-galactosidase-1 (beta-Gal-1) gene has been identified in the 26A7-9 interval.

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Year:  1991        PMID： 1903503     DOI： 10.1007/bf00273609

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  31 in total

1.  Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.

Authors:  S Hayashi; I C Gillam; A D Delaney; G M Tener
Journal:  J Histochem Cytochem       Date:  1978-08       Impact factor: 2.479

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors:  P S Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

4.  The genetic and epigenetic control of sn-glycerol-3-phosphate dehydrogenase isozyme expression during the development of Drosophila melanogaster.

Authors:  G C Bewley
Journal:  Isozymes Curr Top Biol Med Res       Date:  1983

5.  Lambda replacement vectors carrying polylinker sequences.

Authors:  A M Frischauf; H Lehrach; A Poustka; N Murray
Journal:  J Mol Biol       Date:  1983-11-15       Impact factor: 5.469

6.  The isolation of structural genes from libraries of eucaryotic DNA.

Authors:  T Maniatis; R C Hardison; E Lacy; J Lauer; C O'Connell; D Quon; G K Sim; A Efstratiadis
Journal:  Cell       Date:  1978-10       Impact factor: 41.582

7.  Structural characterization of the alpha-glycerol-3-phosphate dehydrogenase-encoding gene of Drosophila melanogaster.

Authors:  L von Kalm; J Weaver; J DeMarco; R J MacIntyre; D T Sullivan
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

8.  A cytogenetic analysis of the chromosomal region surrounding the alpha-glycerophosphate dehydrogenase locus of Drosophila melanogaster.

Authors:  M A Kotarski; S Pickert; R J MacIntyre
Journal:  Genetics       Date:  1983-10       Impact factor: 4.562

9.  Drosophila sn-glycerol-3-phosphate dehydrogenase isozymes are generated by alternate pathways of RNA processing resulting in different carboxyl-terminal amino acid sequences.

Authors:  J L Cook; G C Bewley; J B Shaffer
Journal:  J Biol Chem       Date:  1988-08-05       Impact factor: 5.157

10.  Isolation and chromosomal location of putative vitelline membrane genes in Drosophila melanogaster.

Authors:  M N Mindrinos; L J Scherer; F J Garcini; H Kwan; K A Jacobs; W H Petri
Journal:  EMBO J       Date:  1985-01       Impact factor: 11.598
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  1 in total

1.  Identification of an essential Drosophila gene that is homologous to the translation initiation factor eIF-4A of yeast and mouse.

Authors:  R Dorn; H Morawietz; G Reuter; H Saumweber
Journal:  Mol Gen Genet       Date:  1993-02
  1 in total

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