| Literature DB >> 6261081 |
P Stougaard, S Molin, K Nordström, F G Hansen.
Abstract
The region of plasmid R 1 containing the replication control genes has been sequenced using the Maxam-Gilbert method. The nucleotide sequence of two small PstI restriction fragments (a total of about 1,000 base pairs) was determined for the wild-type R 1 plasmid as well as for two different copy mutants. It was found that one copy mutant has a single base substitution in the fragment which was recently shown to harbor an important inc/cop gene (Molin and Nordström 1980). Furthermore, the sequence indicates the presence of a structural gene that codes for a polypeptide of size 10,500 daltons. Possible gene products predicted from the nucleotide sequences and their role in replication control are discussed.Entities:
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Year: 1981 PMID: 6261081 DOI: 10.1007/bf00339014
Source DB: PubMed Journal: Mol Gen Genet ISSN: 0026-8925