Literature DB >> 6261007

Structure of Bacillus subtilis bacteriophage SPP1 DNA in relation to its transfection activity.

G O Humphreys, T A Trautner.   

Abstract

The availability of a detailed restriction map of SPP1 DNA allowed defined manipulations of such molecules. These were performed to investigate structural requirements for SPP1 transfection. (i) The transfection activity of SPP1 DNA was destroyed by degradation with restriction enzymes. Biological activity could be regenerated when transfection was performed with a combination of two different restriction endonuclease digests, provided that such digests generated widely overlapping DNA fragments. (ii) Unique DNA molecules were constructed from the natural population of circularly permuted SPP1 DNA molecules by using genetic engineering techniques. Such molecules had the same specific transfection activity as did the circularly permuted SPP1 DNA. These results are discussed in the context of current models of DNA processing in transfection.

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Year:  1981        PMID: 6261007      PMCID: PMC171044     

Source DB:  PubMed          Journal:  J Virol        ISSN: 0022-538X            Impact factor:   5.103


  22 in total

1.  Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.

Authors:  M Thomas; R W Davis
Journal:  J Mol Biol       Date:  1975-01-25       Impact factor: 5.469

2.  Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R.

Authors:  S Bron; K Murray; T A Trautner
Journal:  Mol Gen Genet       Date:  1975-12-30

Review 3.  Transfection in B. subtilis.

Authors:  T A Trautner; H C Spatz
Journal:  Curr Top Microbiol Immunol       Date:  1973       Impact factor: 4.291

4.  Mature DNA from temperate bacillusphage phi105 requires primary recombination to be infectious in transfection.

Authors:  J I Flock; L Rutberg
Journal:  Mol Gen Genet       Date:  1974

5.  Restriction and modification in B. subtilis. Biological aspects.

Authors:  T A Trautner; B Pawlek; S Bron; C Anagnostopoulos
Journal:  Mol Gen Genet       Date:  1974

6.  Genetic and transfection studies with B. subtilis phage SP50. 3. Biological effects of DNA cleavage and the physical basis of the map.

Authors:  W R Havender; T A Trautner
Journal:  Mol Gen Genet       Date:  1972

7.  A new phage of Bacillus subtilis with infectious DNA having separable strands.

Authors:  S Riva; M Polsinelli; A Falaschi
Journal:  J Mol Biol       Date:  1968-07-28       Impact factor: 5.469

8.  The role of recombination in transfection of B. subtilis.

Authors:  H C Spatz; T A Trautner
Journal:  Mol Gen Genet       Date:  1971

9.  Genetic and transfection studies with B, subtilis phage SP 50. I. Phage mutants with restricted growth on B. subtilis strain 168.

Authors:  E Rottländer; T A Trautner
Journal:  Mol Gen Genet       Date:  1970

10.  Induction of mutations in B. subtilis phage SPP1 by growth on host cells carrying a mutator DNA polymerase III.

Authors:  M A Montenegro; H Esche; T A Trautner
Journal:  Mol Gen Genet       Date:  1976-12-08
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