Literature DB >> 6260972

Isolation of recombinant DNA clones carrying complete integrated proviruses of Moloney murine leukemia virus.

L Bacheler, H Fan.   

Abstract

EcoRI DNA fragments from a Moloney murine leukemia virus (M-MuLV)-infected mouse fibroblast line (M-MuLV clone A9) were cloned in lambda phage Charon 4A cloning vector to derive clones containing integrated M-MuLV proviral DNA. A 10- to 16-megadalton class of EcoRI fragments was chosen for cloning, based on (i) its ability to induce XC-positive virus upon transfection of NIH/3T3 cells, and (ii) its content of a 0.8-megadalton viral KpnI fragment diagnostic for M-MuLV. Six recombinant DNA clones were isolated which contain a complete M-MuLV provirus, as judged by (i) restriction endonuclease mapping and (ii) the fact that all of the clones gave rise to XC-positive, NB-tropic virus upon DNA infection in NIH/3T3 cells. The sizes of the inserts were 12.0 (for three clones) or 12.5 megadaltons (for three clones). Restriction mapping indicated that these six clones represent five different M-MuLV proviral integrations into different cellular DNA sites.

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Year:  1981        PMID： 6260972      PMCID： PMC170995     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  30 in total

1.  Proviral DNA of Moloney leukemia virus: purification and visualization.

Authors:  A M Gianni; J R Hutton; D Smotkin; R A Weinberg
Journal:  Science       Date:  1976-02-13       Impact factor: 47.728

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Chromosomal subunits in active genes have an altered conformation.

Authors:  H Weintraub; M Groudine
Journal:  Science       Date:  1976-09-03       Impact factor: 47.728

4.  Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors:  W D Benton; R W Davis
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

5.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

6.  Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.

Authors:  F R Blattner; B G Williams; A E Blechl; K Denniston-Thompson; H E Faber; L Furlong; D J Grunwald; D O Kiefer; D D Moore; J W Schumm; E L Sheldon; O Smithies
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

7.  Plaque assay techniques for murine leukemia viruses.

Authors:  W P Rowe; W E Pugh; J W Hartley
Journal:  Virology       Date:  1970-12       Impact factor: 3.616

8.  Infectious DNA of spleen necrosis virus is integrated at a single site in the DNA of chronically infected chicken fibroblasts.

Authors:  N Battula; H M Temin
Journal:  Proc Natl Acad Sci U S A       Date:  1977-01       Impact factor: 11.205

9.  Germ line integration and Mendelian transmission of the exogenous Moloney leukemia virus.

Authors:  R Jaenisch
Journal:  Proc Natl Acad Sci U S A       Date:  1976-04       Impact factor: 11.205

10.  Measurement of the sequence complexity of cloned Moloney murine leukemia virus 60 to 70S RNA: evidence for a haploid genome.

Authors:  H Fan; M Paskind
Journal:  J Virol       Date:  1974-09       Impact factor: 5.103
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  33 in total

1.  Possible role of splice acceptor site in expression of unspliced gag-containing message of Moloney murine leukemia virus.

Authors:  M Oshima; T Odawara; T Matano; H Sakahira; Y Kuchino; A Iwamoto; H Yoshikura
Journal:  J Virol       Date:  1996-04       Impact factor: 5.103

2.  cis Elements required for high-level expression of unspliced Gag-containing message in Moloney murine leukemia virus.

Authors:  M Oshima; T Odawara; K Hanaki; H Igarashi; H Yoshikura
Journal:  J Virol       Date:  1998-08       Impact factor: 5.103

3.  An embryonic DNA-binding protein specific for the promoter of the retrovirus long terminal repeat.

Authors:  F Flamant; C C Gurin; J A Sorge
Journal:  Mol Cell Biol       Date:  1987-10       Impact factor: 4.272

4.  Properties of the naturally occurring soluble surface glycoprotein of ecotropic murine leukemia virus: binding specificity and possible conformational change after binding to receptor.

Authors:  H Ikeda; K Kato; T Suzuki; H Kitani; Y Matsubara; S Takase-Yoden; R Watanabe; M Kitagawa; S Aizawa
Journal:  J Virol       Date:  2000-02       Impact factor: 5.103

5.  Replication-defective chimeric helper proviruses and factors affecting generation of competent virus: expression of Moloney murine leukemia virus structural genes via the metallothionein promoter.

Authors:  R A Bosselman; R Y Hsu; J Bruszewski; S Hu; F Martin; M Nicolson
Journal:  Mol Cell Biol       Date:  1987-05       Impact factor: 4.272

6.  Expression of hepatitis B virus surface and core antigens: influences of pre-S and precore sequences.

Authors:  A McLachlan; D R Milich; A K Raney; M G Riggs; J L Hughes; J Sorge; F V Chisari
Journal:  J Virol       Date:  1987-03       Impact factor: 5.103

7.  Threshold number of provirus copies required per cell for efficient virus production and interference in moloney murine leukemia virus-infected NIH 3T3 cells.

Authors:  T Odawara; M Oshima; K Doi; A Iwamoto; H Yoshikura
Journal:  J Virol       Date:  1998-07       Impact factor: 5.103

8.  Methylation state and DNase I sensitivity of chromatin containing Moloney murine leukemia virus DNA in exogenously infected mouse cells.

Authors:  P E Montandon; F Montandon; H Fan
Journal:  J Virol       Date:  1982-11       Impact factor: 5.103

9.  Complete correction of the enzymatic defect of type I Gaucher disease fibroblasts by retroviral-mediated gene transfer.

Authors:  J Sorge; W Kuhl; C West; E Beutler
Journal:  Proc Natl Acad Sci U S A       Date:  1987-02       Impact factor: 11.205

10.  Cloning of two genetically transmitted Moloney leukemia proviral genomes: correlation between biological activity of the cloned DNA and viral genome activation in the animal.

Authors:  I Chumakov; H Stuhlmann; K Harbers; R Jaenisch
Journal:  J Virol       Date:  1982-06       Impact factor: 5.103
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