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Literature DB >> 9658082

cis Elements required for high-level expression of unspliced Gag-containing message in Moloney murine leukemia virus.

M Oshima¹, T Odawara, K Hanaki, H Igarashi, H Yoshikura.

Abstract

The 441-nucleotide (nt) region (nt 5325 to 5766) around the splice acceptor (SA) site (nt 5491) was found to be necessary for high-level expression of gag-containing unspliced RNA of Moloney murine leukemia virus (M. Oshima, T. Odawara, T. Matano, H. Sakahira, K. Kuchino, A. Iwamoto, and H. Yoshikura, J. Virol. 70:2286-2295, 1996). Detailed genetic dissection of the 441-nt region revealed that the 5'-end 64 nt (nt 5325 to 5389) were necessary for high-level expression of the unspliced RNA when the spliced RNA was not produced, while the 3'-side 301 nt (nt 5466 to 5766) containing the SA site were necessary for producing spliced RNA. When the spliced RNA was produced, the unspliced RNA could be expressed at a high level even when the 5'-end 64 nt were absent. Probably the virus sequence ensuring the splicing could produce an RNA structure able to compensate for the function of the 5'-end 64-nt region responsible for the expression of the unspliced RNA.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1998 PMID： 9658082 PMCID： PMC109795

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

25 in total

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5. Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes.

Authors: R K Saiki; T L Bugawan; G T Horn; K B Mullis; H A Erlich
Journal: Nature Date: 1986 Nov 13-19 Impact factor: 49.962

6. Identification and characterization of intragenic sequences which repress human immunodeficiency virus structural gene expression.

Authors: A W Cochrane; K S Jones; S Beidas; P J Dillon; A M Skalka; C A Rosen
Journal: J Virol Date: 1991-10 Impact factor: 5.103

1. R region sequences in the long terminal repeat of a murine retrovirus specifically increase expression of unspliced RNAs.

Authors: A M Trubetskoy; S A Okenquist; J Lenz
Journal: J Virol Date: 1999-04 Impact factor: 5.103

Review 2. Destiny of unspliced retroviral RNA: ribosome and/or virion?

Authors: Melinda Butsch; Kathleen Boris-Lawrie
Journal: J Virol Date: 2002-04 Impact factor: 5.103

Review 3. The retrovirus RNA trafficking granule: from birth to maturity.

Authors: Alan W Cochrane; Mark T McNally; Andrew J Mouland
Journal: Retrovirology Date: 2006-03-17 Impact factor: 4.602

3 in total