Literature DB >> 6260574

A new high-capacity cosmid vector and its use.

E M Meyerowitz, G M Guild, L S Prestidge, D S Hogness.   

Abstract

The construction of a cosmid, MUA-3, designed for the convenient cloning of eukaryotic DNA segments up to 48 kb in length is described. The cosmid contains all of the plasmid pBR322 with approx. 400 bases of lambda DNA, including the cohesive end site, inserted at the pBR322 PstI endonuclease recognition site. Methods for using this vector to construct several types of Drosophila melanogaster genomic DNA libraries are given, and libraries made by these methods are characterized. A sheared Drosophila DNA-EcoRI linker library is shown to stably maintain average Drosophila DNA inserts of over 40 kb and up to 48 kb, and the efficiency of producing clones by a partial restriction and ligation method is shown to be over 3 X 10(5) clones/microgram of Drosophila DNA.

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Year:  1980        PMID： 6260574     DOI： 10.1016/0378-1119(80)90067-0

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  38 in total

1.  Direct and general selection for lysogens of Escherichia coli by phage lambda recombinant clones.

Authors:  M F Henry; J E Cronan
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

2.  Patents and literature.

Authors:  A Rembaum; R P Rohrbach; S Klose; H G Batz; M Stoltz; A Hagen; G Weimann; S G Wildman; P Kwanyuen; G Franzmann; H L Wulsmann; T Nakanishi; Y Shigemasa; R Monsheimer; E Pfleiderer; T Yoshioka; K Teramoto; M Shimamura; C K Lee; M Tamuri; M Kanno; Y Ishi; I Kaetsu; M Kumakura; M Yoshida; G A Strobel; D E Johnson; V R Srinivasan; Y C Choi; H Fukuda; T Shiotani; W Okada; R A Messing; R A Oppermann; L B Simpson; M M Takeguchi; M Bigazzi; T B Higerd; J M Goust; W R Tolbert; M J Kuo; J Feder; R N Patel; C T Hou; A I Laskin; H Yukawa; K Osumi; Y Takayama; A Fujiwara; S Masuda; F Takaku; K Ogasa; M Kuboyama; M Saito; N Yanai; M Nishida; O Tosaka; E Ono; M Ishihara; H Morioka; K Takinami; A Taoka; S Uchida; J Puls; M Sinner; H H Dietrichs; D T Wicklow; R W Detroy; R E Heady; H Yukawa; K Osumi; T Nara; Y Takayama; E Knight; G T Tsao; M R Ladisch; C M Ladisch; T A Hsu; H J Friesen; S Pestka; J J Manis; V G Debabov; J I Kozlov; N I Zhdanova; E M Khurges; N K Yankovsky; M R Rozinov; R S Shakulov; B A Rebentish; V A Livshits; M M Gusyatiner; S V Mashko; V N Moshentseva; L F Kozyreva; R A Arsatiants; H M Goodman; J Shine; P Horst; D V Goeddel; H L Heyneker
Journal:  Appl Biochem Biotechnol       Date:  1981-12       Impact factor: 2.926

3.  Cloning and sequence of the gene encoding the major structural component of mannose-resistant fimbriae of Serratia marcescens.

Authors:  Y Mizunoe; Y Nakabeppu; M Sekiguchi; S Kawabata; T Moriya; K Amako
Journal:  J Bacteriol       Date:  1988-08       Impact factor: 3.490

4.  A cosmid vector that facilitates restriction enzyme mapping.

Authors:  P F Little; S H Cross
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

5.  Molecular cloning of engrailed: a gene involved in the development of pattern in Drosophila melanogaster.

Authors:  J M Kuner; M Nakanishi; Z Ali; B Drees; E Gustavson; J Theis; L Kauvar; T Kornberg; P H O'Farrell
Journal:  Cell       Date:  1985-08       Impact factor: 41.582

6.  From monoclonal antibody to gene for a neuron-specific glycoprotein in Drosophila.

Authors:  S L Zipursky; T R Venkatesh; S Benzer
Journal:  Proc Natl Acad Sci U S A       Date:  1985-03       Impact factor: 11.205

7.  Extension of the host range of Escherichia coli vectors by incorporation of RSF1010 replication and mobilization functions.

Authors:  U B Priefer; R Simon; A Pühler
Journal:  J Bacteriol       Date:  1985-07       Impact factor: 3.490

8.  Transformation of Aspergillus flavus to study aflatoxin biosynthesis.

Authors:  G A Payne; C P Woloshuk
Journal:  Mycopathologia       Date:  1989-09       Impact factor: 2.574

9.  Cloning of genes determining the production of mannose-resistant fimbriae in a uropathogenic strain of Escherichia coli belonging to serogroup O6.

Authors:  S Clegg
Journal:  Infect Immun       Date:  1982-11       Impact factor: 3.441

10.  The construction of cosmid libraries which can be used to transform eukaryotic cells.

Authors:  F G Grosveld; T Lund; E J Murray; A L Mellor; H H Dahl; R A Flavell
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971
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