Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A sequence-specific endonuclease (Xmn I) from Xanthomonas manihotis.

Literature DB >> 6258165

A sequence-specific endonuclease (Xmn I) from Xanthomonas manihotis.

Abstract

A type II restriction endonuclease Xmn I with a novel site specificity has been isolated from Xanthomonas manihotis. Xmn I does not cleave SV40 DNA, but cleaves phi X174 DNA into three fragments, which constitute 76.61%, 18.08% and 5.31% of the total length of 5386 base pairs, and cleaves pBR322 DNA into two fragments of 55.71% and 44.29% of the entire 4362 base pairs. The nucleotide sequences around the cleavage sites made by Xmn I are not exactly homologous, but they have a common sequence of 5' GAANNNNTTC 3' according to a simple computer program analysis on nucleotide sequences of phi X174 DNA, pBR322 DNA and SV40 DNA. The results suggest that the cleavage site of Xmn I is located within its recognition sequence of 5' GAANNNNTTC 3'.

Entities: Species

Mesh：

Substances：

Year: 1980 PMID： 6258165 PMCID： PMC328081 DOI： 10.1093/nar/8.24.6189

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

24 in total

1. Altering the specificity of restriction endonuclease: effect of replacing Mg2+ with Mn2+.

Authors: M Hsu; P Berg
Journal: Biochemistry Date: 1978-01-10 Impact factor: 3.162

2. Two restriction-like enzymes from Xanthomonas malvacearum.

Authors: S A Endow; R J Roberts
Journal: J Mol Biol Date: 1977-05-25 Impact factor: 5.469

3. In vivo site-specific genetic recombination promoted by the EcoRI restriction endonuclease.

Authors: S Chang; S N Cohen
Journal: Proc Natl Acad Sci U S A Date: 1977-11 Impact factor: 11.205

4. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors: D B Clewell
Journal: J Bacteriol Date: 1972-05 Impact factor: 3.490

5. Deletion mutants of simian virus 40 generated by enzymatic excision of DNA segments from the viral genome.

Authors: C J Lai; D Nathans
Journal: J Mol Biol Date: 1974-10-15 Impact factor: 5.469

6. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.

Authors: R Radloff; W Bauer; J Vinograd
Journal: Proc Natl Acad Sci U S A Date: 1967-05 Impact factor: 11.205

7. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

Authors: M W McDonell; M N Simon; F W Studier
Journal: J Mol Biol Date: 1977-02-15 Impact factor: 5.469

Review 8. Restriction endonucleases.

Authors: R J Roberts
Journal: CRC Crit Rev Biochem Date: 1976-11

9. EcoRI endonuclease. Physical and catalytic properties of the homogenous enzyme.

Authors: P Modrich; D Zabel
Journal: J Biol Chem Date: 1976-10-10 Impact factor: 5.157

10. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.

Authors: J A Meyers; D Sanchez; L P Elwell; S Falkow
Journal: J Bacteriol Date: 1976-09 Impact factor: 3.490

8 in total

A sequence-specific endonuclease (Xmn I) from Xanthomonas manihotis.

1. Altering the specificity of restriction endonuclease: effect of replacing Mg2+ with Mn2+.

2. Two restriction-like enzymes from Xanthomonas malvacearum.

3. In vivo site-specific genetic recombination promoted by the EcoRI restriction endonuclease.

4. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

5. Deletion mutants of simian virus 40 generated by enzymatic excision of DNA segments from the viral genome.

6. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.

7. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

Review 8. Restriction endonucleases.

9. EcoRI endonuclease. Physical and catalytic properties of the homogenous enzyme.

10. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.

1. OliI, a unique restriction endonuclease that recognizes the discontinuous sequence 5'-CACNN NGTG-3'.

2. Restriction enzymes and their isoschizomers.

3. Restriction enzymes and their isoschizomers.

4. Asp700I, a novel isoschizomer of XmnI from Achromobacter species 700 recognizing 5'-GAANN/NNTTC-3'.

5. Restriction and modification enzymes and their recognition sequences.

6. Restriction enzymes and their isoschizomers.

7. Macromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli.

8. Restriction and modification enzymes and their recognition sequences.