Literature DB >> 6258163

Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex.

M D Been, J J Champoux.   

Abstract

The DNA nicking-closing enzyme (type I topoisomerase) from rat liver nuclei breaks single-stranded DNA. The broken strand contains a 5'-hydroxyl and tightly bound protein. The stability of this protein-DNA complex to high salt, alkali and detergent suggests a covalent linkage between the DNA and the enzyme. The observed breakage of single-stranded DNA occurs at neutral pH prior to treatment with alkali or detergent, indicating that the breakage may be the result of an interrupted nicking and closing cycle. The resulting covalent complex could represent a reaction intermediate in the overall nicking-closing reaction.

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Year:  1980        PMID： 6258163      PMCID： PMC328077          DOI： 10.1093/nar/8.24.6129

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  20 in total

1.  Evidence for an intermediate with a single-strand break in the reaction catalyzed by the DNA untwisting enzyme.

Authors:  J J Champoux
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

2.  Assay of RNA-linked nascent DNA pieces with polynucleotide kinase.

Authors:  R Okazaki; S Hirose; T Okazaki; T Ogawa; Y Kurosawa
Journal:  Biochem Biophys Res Commun       Date:  1975-02-17       Impact factor: 3.575

3.  Priming of superhelical SV40 DNA by Escherichia coli RNA polymerase for in vitro DNA synthesis.

Authors:  J J Champoux; B L McConaughy
Journal:  Biochemistry       Date:  1975-01-28       Impact factor: 3.162

4.  Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA.

Authors:  J J Champoux
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

5.  Purification and characterization of the DNA untwisting enzyme from rat liver.

Authors:  J J Champoux; B L McConaughy
Journal:  Biochemistry       Date:  1976-10-19       Impact factor: 3.162

6.  An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).

Authors:  J J Champoux; R Dulbecco
Journal:  Proc Natl Acad Sci U S A       Date:  1972-01       Impact factor: 11.205

7.  Characterization of the replicative intermediates of polyoma virus.

Authors:  A Roman; J J Champoux; R Dulbecco
Journal:  Virology       Date:  1974-01       Impact factor: 3.616

8.  Interaction between DNA and an Escherichia coli protein omega.

Authors:  J C Wang
Journal:  J Mol Biol       Date:  1971-02-14       Impact factor: 5.469

9.  Knotted single-stranded DNA rings: a novel topological isomer of circular single-stranded DNA formed by treatment with Escherichia coli omega protein.

Authors:  L F Liu; R E Depew; J C Wang
Journal:  J Mol Biol       Date:  1976-09-15       Impact factor: 5.469

10.  phiX174 cistron A protein is a multifunctional enzyme in DNA replication.

Authors:  S Eisenberg; J Griffith; A Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205
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  12 in total

1.  Similarity in the catalysis of DNA breakage and rejoining by type IA and IIA DNA topoisomerases.

Authors:  Q Liu; J C Wang
Journal:  Proc Natl Acad Sci U S A       Date:  1999-02-02       Impact factor: 11.205

Review 2.  Topoisomerases as anticancer targets.

Authors:  Justine L Delgado; Chao-Ming Hsieh; Nei-Li Chan; Hiroshi Hiasa
Journal:  Biochem J       Date:  2018-01-23       Impact factor: 3.857

3.  DNA breakage and closure by rat liver type 1 topoisomerase: separation of the half-reactions by using a single-stranded DNA substrate.

Authors:  M D Been; J J Champoux
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

4.  Nucleotide sequence preference at rat liver and wheat germ type 1 DNA topoisomerase breakage sites in duplex SV40 DNA.

Authors:  M D Been; R R Burgess; J J Champoux
Journal:  Nucleic Acids Res       Date:  1984-04-11       Impact factor: 16.971

5.  A destabilized DNA conformation associated with tightly bound nuclear proteins in active genes of rat myoblast.

Authors:  S A Leibovitch; M P Leibovitch; J Hillion; J Kruh; J Harel
Journal:  Nucleic Acids Res       Date:  1983-06-25       Impact factor: 16.971

6.  The A* protein of phi X174 is an inhibitor of DNA replication.

Authors:  S Eisenberg; R Ascarelli
Journal:  Nucleic Acids Res       Date:  1981-04-24       Impact factor: 16.971

7.  Adenovirus infection elevates levels of cellular topoisomerase I.

Authors:  K C Chow; G D Pearson
Journal:  Proc Natl Acad Sci U S A       Date:  1985-04       Impact factor: 11.205

8.  A model for the mechanism of precise integration of a microinjected transgene.

Authors:  M McFarlane; J B Wilson
Journal:  Transgenic Res       Date:  1996-05       Impact factor: 2.788

9.  Mechanistic study of E. coli DNA topoisomerase I: cleavage of oligonucleotides.

Authors:  Y C Tse-Dinh; B G McCarron; R Arentzen; V Chowdhry
Journal:  Nucleic Acids Res       Date:  1983-12-20       Impact factor: 16.971

10.  Cryptic loxP sites in mammalian genomes: genome-wide distribution and relevance for the efficiency of BAC/PAC recombineering techniques.

Authors:  S Semprini; T J Troup; N Kotelevtseva; K King; J R E Davis; L J Mullins; K E Chapman; D R Dunbar; J J Mullins
Journal:  Nucleic Acids Res       Date:  2007-02-06       Impact factor: 16.971
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