Literature DB >> 6254695

Use of Sep-pak cartridges for urinary steroid extraction: evaluation of the method for use prior to gas chromatographic analysis.

C H Shackleton, J O Whitney.   

Abstract

A method is described for the rapid and quantitative extraction of free and conjugated steroids from urine using Sep-pak C18 cartridges. The method was evaluated by determining the efficiency of recovery of (1) radiolabeled steroid glucuronides, (2) radiolabeled steroids freed by enzymatic hydrolysis, (3) steroid sulphates, (4) selected reference neutral free steroids of varied structure and polarity, and (5) oestrogens. In all cases the cartridges gave results equal to or better than those obtained by solvent or Amberlite XAD-2 extraction methods. Each urine extraction could be completed in 2-3 minutes and no further purification of extracts was required prior to derivatisation and gas chromatographic analysis.

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Year:  1980        PMID： 6254695     DOI： 10.1016/0009-8981(80)90451-9

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  18 in total

1.  Biochemical aspects of congenital adrenal hyperplasia.

Authors:  J Honour
Journal:  J Inherit Metab Dis       Date:  1986       Impact factor: 4.982

2.  Urinary excretion of 17-hydroxypregnanolones in patients with different forms of congenital adrenal hyperplasia due to steroid 21-hydroxylase deficiency.

Authors:  J Homoki; J Sólyom; U Wachter; W M Teller
Journal:  Eur J Pediatr       Date:  1992-01       Impact factor: 3.183

Review 3.  Role of a disordered steroid metabolome in the elucidation of sterol and steroid biosynthesis.

Authors:  Cedric H L Shackleton
Journal:  Lipids       Date:  2011-08-27       Impact factor: 1.880

4.  [Urinary steroid profile in Cushing syndrome and in tumors of the adrenal cortex].

Authors:  J Homoki; R Holl; W M Teller
Journal:  Klin Wochenschr       Date:  1987-08-03

5.  The occurrence of estrone and estriol in Trichostrongylus colubriformis (Nematoda).

Authors:  H M Lee; E J Parish; L W Bone
Journal:  Lipids       Date:  1989-10       Impact factor: 1.880

6.  Severe hypoaldosteronism due to corticosterone methyl oxidase type II deficiency in two boys: metabolic and gas chromatography-mass spectrometry studies.

Authors:  B P Hauffa; J Sólyom; E Gláz; C H Shackleton; G Wambach; P Vecsei; H Stolecke; J Homoki
Journal:  Eur J Pediatr       Date:  1991-01       Impact factor: 3.183

7.  Abnormal steroid excretion in gestational trophoblastic disease complicated by ovarian theca-lutein cysts.

Authors:  B R Bevan; M Savvas; J M Jenkins; K Baker; G W Pennington; N F Taylor
Journal:  J Clin Pathol       Date:  1986-06       Impact factor: 3.411

8.  Detection of late onset steroid 21-hydroxylase deficiency by capillary gas chromatographic profiling of urinary steroids in children and adolescents.

Authors:  J Homoki; J Solyom; W M Teller
Journal:  Eur J Pediatr       Date:  1988-04       Impact factor: 3.183

9.  Steroid biochemistry of virilising adrenal tumours in childhood.

Authors:  J W Honour; D A Price; N F Taylor; H B Marsden; D B Grant
Journal:  Eur J Pediatr       Date:  1984-08       Impact factor: 3.183

10.  The definitive identification of the lignans trans-2,3-bis(3-hydroxybenzyl)-gamma-butyrolactone and 2,3-bis(3-hydroxybenzyl)butane-1,4-diol in human and animal urine.

Authors:  K D Setchell; A M Lawson; E Conway; N F Taylor; D N Kirk; G Cooley; R D Farrant; S Wynn; M Axelson
Journal:  Biochem J       Date:  1981-08-01       Impact factor: 3.857
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