Literature DB >> 6253913

Fusion of the promoter region of rRNA operon rrnB to lac Z gene.

G Glaser, S Kobi, A B Oppenheim.   

Abstract

A Lambda phage was constructed in which the structural gene for beta galactosidase is fused to a DNA segment carrying the ribosomal promoter rrnB of E. coli. In this hybrid operon beta galactosidase synthesis in vitro is repressed by ppGpp. Repression of beta galactosidase synthesis by cAMP is reported.

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Year:  1980        PMID： 6253913      PMCID： PMC324242          DOI： 10.1093/nar/8.19.4327

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  18 in total

1.  Genetic fusions defining trp and lac operon regulatory elements.

Authors:  D H Mitchell; W S Reznikoff; J R Beckwith
Journal:  J Mol Biol       Date:  1975-04-15       Impact factor: 5.469

2.  Isolation, characterization and deletion mapping of amber mutations in the cll gene of phage lambda.

Authors:  M Belfort; D Noff; A B Oppenheim
Journal:  Virology       Date:  1975-01       Impact factor: 3.616

3.  Regulation of bacterial ppGpp and pppGpp.

Authors:  M Cashel
Journal:  Annu Rev Microbiol       Date:  1975       Impact factor: 15.500

4.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

Review 5.  Cyclic adenosine 5'-monophosphate in Escherichia coli.

Authors:  I Pastan; S Adhya
Journal:  Bacteriol Rev       Date:  1976-09

Review 6.  Genetics of bacterial ribosomes.

Authors:  M Nomura; E A Morgan
Journal:  Annu Rev Genet       Date:  1977       Impact factor: 16.830

7.  The construction of lambda transducing phages containing deletions defining regulatory elements of the lac and trp operons in E. coli.

Authors:  W M Barnes; R B Siegel; W S Reznikoff
Journal:  Mol Gen Genet       Date:  1974-03-27

Review 8.  In vitro synthesis of protein in microbial systems.

Authors:  G Zubay
Journal:  Annu Rev Genet       Date:  1973       Impact factor: 16.830

9.  Modulation of RNA polymerase specificity by ppGpp.

Authors:  A Travers
Journal:  Mol Gen Genet       Date:  1976-08-19

10.  Effects of guanosine tetraphosphate, guanosine pentaphosphate, and beta-gamma methylenyl-guanosine pentaphosphate on gene expression of Escherichia coli in vitro.

Authors:  H L Yang; G Zubay; E Urm; G Heiness; M Cashel
Journal:  Proc Natl Acad Sci U S A       Date:  1974-01       Impact factor: 11.205
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  1 in total

1.  Construction and characterization of a tufA-lacZ fusion coding for an E. coli EF-Tu-beta-galactosidase chimeric protein.

Authors:  K Gausing
Journal:  Mol Gen Genet       Date:  1981
  1 in total

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