Literature DB >> 6251092

Transcription and in vitro processing of yeast 5 S rRNA.

P A Tekamp, R L Garcea, W J Rutter.   

Abstract

A method is described for the isolation of a yeast chromatin fraction highly enriched in ribosomal DNA sequences. In the presence of exogenous yeast RNA polymerase III, this purified chromatin actively synthesizes a set of 5 S ribosomal RNAs all of which have 5'-sequences identical with mature 5 S RNA but which end with a variable number (up to 10) of additional residues at the 3'-terminus. These extra nucleotides are precisely removed by a processing nuclease found in the chromatin supernatant fraction.

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Year:  1980        PMID： 6251092

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  8 in total

1.  Selective and accurate initiation of transcription at the T-DNA promoter in a soluble chromatin extract from wheat germ.

Authors:  K Yamazaki; F Imamoto
Journal:  Mol Gen Genet       Date:  1987-10

2.  Nuclear RNA surveillance in Saccharomyces cerevisiae: Trf4p-dependent polyadenylation of nascent hypomethylated tRNA and an aberrant form of 5S rRNA.

Authors:  Sujatha Kadaba; Xuying Wang; James T Anderson
Journal:  RNA       Date:  2006-01-23       Impact factor: 4.942

3.  Nucleotide sequence of small polyadenylated B2 RNA.

Authors:  D A Kramerov; S V Tillib; A P Ryskov; G P Georgiev
Journal:  Nucleic Acids Res       Date:  1985-09-25       Impact factor: 16.971

4.  Yeast exosome mutants accumulate 3'-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs.

Authors:  A van Hoof; P Lennertz; R Parker
Journal:  Mol Cell Biol       Date:  2000-01       Impact factor: 4.272

5.  Transcription initiation in eukaryotes: analysis of heterologous in vitro systems utilizing components from mammalian and yeast cells.

Authors:  G A Bitter
Journal:  Mol Gen Genet       Date:  1983

6.  A yeast transcription system for the 5S rRNA gene.

Authors:  H van Keulen; D Y Thomas
Journal:  Nucleic Acids Res       Date:  1982-09-11       Impact factor: 16.971

7.  Altered maturation of sequences at the 3' terminus of 5S gene transcripts in a Saccharomyces cerevisiae mutant that lacks a RNA processing endonuclease.

Authors:  P W Piper; J A Bellatin; A Lockheart
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

8.  Ribosomal DNA status inferred from DNA cloud assays and mass spectrometry identification of agarose-squeezed proteins interacting with chromatin (ASPIC-MS).

Authors:  Kamil Krol; Justyna Jendrysek; Janusz Debski; Marek Skoneczny; Anna Kurlandzka; Joanna Kaminska; Michal Dadlez; Adrianna Skoneczna
Journal:  Oncotarget       Date:  2017-04-11
  8 in total

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