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Literature DB >> 6248852

Fate of donor insertion sequence IS1 during transposition.

Abstract

The number of insertion sequence IS1 segments in Escherichia coli K-12 and 20 mutants in which an ISI had been inserted at a new site was measured by Southern blot hybridization analysis. The parent strain appeared to contain seven IS1 segments. Each of the mutants contained these seven IS1 and one additional IS1 corresponding to the new IS1 insertion. These results suggest that a copy of a donor ISI is inserted at the new site. A model for transposition is prevented that postulates that a reactive intermediate is formed by a tandem duplication of a transposable sequence.

Entities: Gene Species

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Year: 1980 PMID： 6248852 PMCID： PMC349431 DOI： 10.1073/pnas.77.5.2514

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

33 in total

1. Hybridization of labeled RNA to DNA in agarose gels.

Authors: T M Shinnick; E Lund; O Smithies; F R Blattner
Journal: Nucleic Acids Res Date: 1975-10 Impact factor: 16.971

2. Model for the enchancement of lambde-gal integration into partially induced Mu-1 lysogens.

Authors: M Faelen; A Toussaint; J De Lafonteyne
Journal: J Bacteriol Date: 1975-03 Impact factor: 3.490

3. Isolation of polar insertion mutants and the direction of transcription of ribosomal protein genes in E. coli.

Authors: S R Jaskunas; L Lindahl; M Nomura
Journal: Nature Date: 1975-07-17 Impact factor: 49.962

4. Acquisition of a determinant for chloramphenicol resistance by coliphage lambda.

Authors: M M Gottesman; J L Rosner
Journal: Proc Natl Acad Sci U S A Date: 1975-12 Impact factor: 11.205

5. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

6. Translocatable elements in procaryotes.

Authors: N Kleckner
Journal: Cell Date: 1977-05 Impact factor: 41.582

7. Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K-12.

Authors: H Saedler; B Heiss
Journal: Mol Gen Genet Date: 1973-05-09

Review 8. Controlling elements in maize.

Authors: J R Fincham; G R Sastry
Journal: Annu Rev Genet Date: 1974 Impact factor: 16.830

9. Organization of ribosomal protein genes in Escherichia coli. I. Physical structure of DNA from transducing lambda phages carrying genes from the aroE-str region.

Authors: M Fiandt; W Szybalski; F R Blattner; S R Jaskunas; L Lindahl; M Nomura
Journal: J Mol Biol Date: 1976-09-25 Impact factor: 5.469

10. IS1 and IS2 mutations in the ribosomal protein genes of E. coli K-12.

Authors: H Saedler; D F Kubai; M Nomura; S R Jaskunas
Journal: Mol Gen Genet Date: 1975-11-03

7 in total

1. Structure of the DNA distal to the gene for ribosomal protein S20 in Escherichia coli K12: presence of a strong terminator and an IS1 element.

Authors: G A Mackie
Journal: Nucleic Acids Res Date: 1986-09-11 Impact factor: 16.971

Fate of donor insertion sequence IS1 during transposition.

1. Hybridization of labeled RNA to DNA in agarose gels.

2. Model for the enchancement of lambde-gal integration into partially induced Mu-1 lysogens.

3. Isolation of polar insertion mutants and the direction of transcription of ribosomal protein genes in E. coli.

4. Acquisition of a determinant for chloramphenicol resistance by coliphage lambda.

5. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

6. Translocatable elements in procaryotes.

7. Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K-12.

Review 8. Controlling elements in maize.

9. Organization of ribosomal protein genes in Escherichia coli. I. Physical structure of DNA from transducing lambda phages carrying genes from the aroE-str region.

10. IS1 and IS2 mutations in the ribosomal protein genes of E. coli K-12.

1. Structure of the DNA distal to the gene for ribosomal protein S20 in Escherichia coli K12: presence of a strong terminator and an IS1 element.

2. Recombination in recA cells between direct repeats of insertion element IS1.

3. Isolation of E. coli mutants containing multiple transpositions of IS sequences.

4. Mapping of chromosomal IS5 elements that mediate type II F-prime plasmid excision in Escherichia coli K-12.

5. Genetic structure, function and regulation of the transposable element IS21.

6. A transposable element from Halobacterium halobium which inactivates the bacteriorhodopsin gene.

7. Mapping of IS1 elements flanking the argF gene region on the Escherichia coli K-12 chromosome.