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Literature DB >> 6255292

Isolation of E. coli mutants containing multiple transpositions of IS sequences.

Abstract

The characterization of three E. coli mutants that appeared to have unselected IS1 insertions on the chromosome are described. One had a single new IS1 sequence. The second had three new IS1 sequences. The third had two new IS1 sequences and one of the IS1 sequences in the parent was missing. These mutants were found in a collection on strains that contained IS insertions in the spc operon. The frequency of finding mutants with unselected IS1 transpositions was at least 100 times greater than expected. The results suggest several transposition events may frequently occur in the same cell.

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Year: 1980 PMID： 6255292 DOI： 10.1007/bf00267365

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

39 in total

9. R factor-mediated tetracycline resistance in Escherichia coli K12. Dominance of some tetracycline sensitive mutants and relief of dominance by deletion.

Authors: T J Foster
Journal: Mol Gen Genet Date: 1976-02-02

Isolation of E. coli mutants containing multiple transpositions of IS sequences.

1. Hybridization of labeled RNA to DNA in agarose gels.

2. Model for the enchancement of lambde-gal integration into partially induced Mu-1 lysogens.

3. Analysis of sequences transposed by complementation of two classes of transposition-deficient mutants of Tn3.

4. Nucleotide sequence of the transposable DNA-element IS2.

5. Polar mutations in lac, gal and phage lambda consist of a few IS-DNA sequences inserted with either orientation.

6. Mutations of coliphage lambda affecting the expression of replicative functions O and P.

7. O0 and strong-polar mutations in the gal operon are insertions.

8. Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements.

9. R factor-mediated tetracycline resistance in Escherichia coli K12. Dominance of some tetracycline sensitive mutants and relief of dominance by deletion.

10. Translocation specificity of the Tn3 element: characterization of sites of multiple insertions.

1. Construction of stable, single-copy luciferase gene fusions in Escherichia coli.

2. Gamma delta-mediated deletions of chromosomal segments on F-prime plasmids.

3. The ancestry of insertion sequences common to Escherichia coli and Salmonella typhimurium.