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Literature DB >> 6247613

Organization of the nuclear ribosomal DNA of Chlamydomonas reinhardii.

Abstract

Hybridization of cytoplasmic ribosomal RNA (rRNA) to restriction endonuclease digests of nuclear DNA of Chlamydomonas reinhardii reveals two BamHI ribosomal fragments of 2.95 and 2.35 x 10(6) d and two SalI ribosomal fragments of 3.8 and 1.5 x 10(6) d. The ribosomal DNA (rDNA) units 5.3 x 10(6) d in size, appear to be homogeneous since no hybridization of rDNA to other nuclear DNA fragments can be detected. The two BamHI and SalI ribosomal fragments have been cloned and a restriction map of the ribosomal unit has been established. The location of the 25S, 18S and 5.8S rRNA genes has been determined by hybridizing the rRNAs to digests of the ribosomal fragments and by observing RNA/DNA duplexes in the electron microscope. The data also indicate that the rDNA units are arranged in tandem arrays. The 5S rRNA genes are not closely located to the 25S and 18S rRNA genes since they are not contained within the nuclear rDNA unit. In addition no sequence homology is detectable between the nuclear and chloroplast rDNA units of C. reinhardii.

Mesh：

Substances：

Year: 1980 PMID： 6247613 DOI： 10.1007/bf00272684

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

38 in total

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors: T Maniatis; A Jeffrey; H van deSande
Journal: Biochemistry Date: 1975-08-26 Impact factor: 3.162

2. Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.

Authors: G I Bell; L J DeGennaro; D H Gelfand; R J Bishop; P Valenzuela; W J Rutter
Journal: J Biol Chem Date: 1977-11-25 Impact factor: 5.157

3. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

4. Arrangement of the 5-8 S RNA cistrons in the genome of Xenopus laevis.

Authors: J Speirs; M Birnstiel
Journal: J Mol Biol Date: 1974-08-05 Impact factor: 5.469

5. Organization of the ribosomal RNA genes of Dictyostelium discoideum. Mapping of the transcribed region.

Authors: G Frankel; A F Cockburn; K L Kindle; R A Firtel
Journal: J Mol Biol Date: 1977-02-05 Impact factor: 5.469

6. The molecular basis for length heterogeneity in ribosomal DNA from Xenopus laevis.

Authors: P K Wellauer; I B Dawid; D D Brown; R H Reeder
Journal: J Mol Biol Date: 1976-08-25 Impact factor: 5.469

7. Homology between nucleic acids of blue-green algae and chloroplasts of Euglena gracilis.

Authors: G H Pigott; N G Carr
Journal: Science Date: 1972-03-17 Impact factor: 47.728

8. Restriction endonuclease analysis of ribosomal DNA from Saccharomyces cerevisiae.

Authors: J H Cramer; F W Farrelly; R H Rownd
Journal: Mol Gen Genet Date: 1976-11-17

9. The 5S genes of Drosophila melanogaster.

Authors: S Artavanis-Tsakonas; P Schedl; C Tschudi; V Pirrotta; R Steward; W J Gehring
Journal: Cell Date: 1977-12 Impact factor: 41.582

10. The structural organization of ribosomal DNA in Drosophila melanogaster.

Authors: P K Wellauer; I B Dawid
Journal: Cell Date: 1977-02 Impact factor: 41.582

22 in total

1. A plausible model for reversal of neoplastic transformations in plants based on multiple steady states.

Authors: J F Hervagault; P J Ortoleva; J Ross
Journal: Proc Natl Acad Sci U S A Date: 1991-12-01 Impact factor: 11.205

2. Characterization of transcribed dispersed repetitive DNAs in the nuclear genome of the green alga Chlamydomonas reinhardtii.

Authors: A Day; J D Rochaix
Journal: Curr Genet Date: 1989-09 Impact factor: 3.886

7. An evaluation of the phylogenetic position of the dinoflagellate Crypthecodinium cohnii based on 5S rRNA characterization.

Authors: A G Hinnebusch; L C Klotz; R L Blanken; A R Loeblich
Journal: J Mol Evol Date: 1981 Impact factor: 2.395

Organization of the nuclear ribosomal DNA of Chlamydomonas reinhardii.

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

2. Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.

3. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

4. Arrangement of the 5-8 S RNA cistrons in the genome of Xenopus laevis.

5. Organization of the ribosomal RNA genes of Dictyostelium discoideum. Mapping of the transcribed region.

6. The molecular basis for length heterogeneity in ribosomal DNA from Xenopus laevis.

7. Homology between nucleic acids of blue-green algae and chloroplasts of Euglena gracilis.

8. Restriction endonuclease analysis of ribosomal DNA from Saccharomyces cerevisiae.

9. The 5S genes of Drosophila melanogaster.

10. The structural organization of ribosomal DNA in Drosophila melanogaster.

1. A plausible model for reversal of neoplastic transformations in plants based on multiple steady states.

2. Characterization of transcribed dispersed repetitive DNAs in the nuclear genome of the green alga Chlamydomonas reinhardtii.

3. Bryophyte 5S rDNA was inserted into 45S rDNA repeat units after the divergence from higher land plants.

4. Phylogenetic relationships between chlorophytes, chrysophytes, and oomycetes.

5. Portrait of a species: Chlamydomonas reinhardtii.

6. Insertional mutants of Chlamydomonas reinhardtii that require elevated CO(2) for survival.

7. An evaluation of the phylogenetic position of the dinoflagellate Crypthecodinium cohnii based on 5S rRNA characterization.

8. Comparison of the nuclear ribosomal units of five Chlamydomonas species.

9. Large arrays of tandemly repeated DNA sequences in the green alga Chlamydomonas reinhardtii.

10. The organization of macronuclear rDNA molecules of four hypotrichous ciliated protozoans.