| Literature DB >> 6247335 |
M Spector, S O'Neal, E Racker.
Abstract
Plasma membranes from Ehrlich ascites tumor cells were solubilized by octylglucoside in the presence of phospholipids. The Na+K+-ATPase was purified from this extract by adsorption and elution from thio-Seph-arose 4B. The enzyme (specific activity, 7 mumoles of ATP hydrolyzed min-1 mg of protein -1) was reconstituted into liposomes by the octyglucoside dilution procedure. An ATP-dependent Na+ influx with low efficiency was observed. On addition of appropriate amounts of quercetin, the Na+ flux/ATP hydrolysis ratio was increased from 0.4 to 1.4.Entities:
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Year: 1980 PMID： 6247335
Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157