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Literature DB >> 6247319

RsaI: a new sequence-specific endonuclease activity from Rhodopseudomonas sphaeroides.

S P Lynn, L K Cohen, S Kaplan, J F Gardner.

Abstract

A new type II sequence-specific endonuclease, RsaI, has been identified from Rhodopseudomonas sphaeroides strain 28/5. An RsaI purification scheme that yields enzyme which is free of contaminating exonuclease and phosphatase activities after a single column fractionation has been developed. The enzyme recognized the tetranucleotide sequence 5'-GTAC-3' and cleaved between the T and A, thereby generating flush ends. RsaI should be extremely useful in deoxyribonucleic acid sequencing experiments.

Entities: Chemical Species

Mesh：

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Year: 1980 PMID： 6247319 PMCID： PMC293981 DOI： 10.1128/jb.142.2.380-383.1980

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

22 in total

1. Hybridization of labeled RNA to DNA in agarose gels.

Authors: T M Shinnick; E Lund; O Smithies; F R Blattner
Journal: Nucleic Acids Res Date: 1975-10 Impact factor: 16.971

2. A specific endonuclease from Haemophilus haemolyticus.

Authors: R J Roberts; P A Myers; A Morrison; K Murray
Journal: J Mol Biol Date: 1976-05-05 Impact factor: 5.469

3. The mapping and sequence determination of the single site in phiX174am3 replicative form DNA cleaved by restriction endonuclease Pst I.

Authors: N L Brown; M Smith
Journal: FEBS Lett Date: 1976-06-15 Impact factor: 4.124

4. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

5. A thermostable sequence-specific endonuclease from Thermus aquaticus.

Authors: S Sato; C A Hutchinson; J I Harris
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

6. Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z.

Authors: J H Middleton; M H Edgell; C A Hutchison
Journal: J Virol Date: 1972-07 Impact factor: 5.103

7. 'Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction endonucleases.

Authors: R W Blakesley; R D Wells
Journal: Nature Date: 1975-10-02 Impact factor: 49.962

Review 8. Restriction endonucleases.

Authors: R J Roberts
Journal: CRC Crit Rev Biochem Date: 1976-11

9. Characterization of a mini-ColC1 plasmid.

Authors: V Hershfield; H W Boyer; L Chow; D R Helinski
Journal: J Bacteriol Date: 1976-04 Impact factor: 3.490

10. Studies on polynucleotides. LXIX. Synthetic deoxyribopolynucleotides as templates for the DNA polymerase of Escherichia coli: DNA-like polymers containing repeating trinucleotide sequences.

Authors: R D Wells; T M Jacob; S A Narang; H G Khorana
Journal: J Mol Biol Date: 1967-07-28 Impact factor: 5.469

19 in total

1. Molecular cloning and characterization of the gene encoding the adenine methyltransferase M.CviRI from Chlorella virus XZ-6E.

Authors: C Stefan; Y N Xia; J L Van Etten
Journal: Nucleic Acids Res Date: 1991-01-25 Impact factor: 16.971

9. The nucleotide sequence of aroG, the gene for 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase (phe) in Escherichia coli K12.

Authors: W D Davies; B E Davidson
Journal: Nucleic Acids Res Date: 1982-07-10 Impact factor: 16.971

10. Restriction enzymes and their isoschizomers.

Authors: R J Roberts
Journal: Nucleic Acids Res Date: 1988 Impact factor: 16.971

RsaI: a new sequence-specific endonuclease activity from Rhodopseudomonas sphaeroides.

1. Hybridization of labeled RNA to DNA in agarose gels.

2. A specific endonuclease from Haemophilus haemolyticus.

3. The mapping and sequence determination of the single site in phiX174am3 replicative form DNA cleaved by restriction endonuclease Pst I.

4. A new method for sequencing DNA.

5. A thermostable sequence-specific endonuclease from Thermus aquaticus.

6. Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z.

7. 'Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction endonucleases.

Review 8. Restriction endonucleases.

9. Characterization of a mini-ColC1 plasmid.

10. Studies on polynucleotides. LXIX. Synthetic deoxyribopolynucleotides as templates for the DNA polymerase of Escherichia coli: DNA-like polymers containing repeating trinucleotide sequences.

1. Molecular cloning and characterization of the gene encoding the adenine methyltransferase M.CviRI from Chlorella virus XZ-6E.

2. Restriction enzymes and their isoschizomers.

3. Restriction enzymes and their isoschizomers.

4. The cleavage site of the RsaI isoschizomer, CviII, is G decreases TAC.

5. Restriction and modification enzymes and their recognition sequences.

6. A new site-specific endonuclease, ScaI, from Streptomyces caespitosus.

7. Genetic and molecular analysis of aroL, the gene for shikimate kinase II in Escherichia coli K-12.

8. Nucleotide sequence of the transcription unit containing the aroL and aroM genes from Escherichia coli K-12.

9. The nucleotide sequence of aroG, the gene for 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase (phe) in Escherichia coli K12.

10. Restriction enzymes and their isoschizomers.