Literature DB >> 2998344

A new site-specific endonuclease, ScaI, from Streptomyces caespitosus.

H Takahashi, H Kojima, H Saito.   

Abstract

A new site-specific endonuclease has been isolated from Streptomyces caespitosus and named ScaI. Based on analysis of sequences around the restriction sites in pBR322 and pBR325, the recognition sequence of ScaI endonuclease was deduced to be a new hexanucleotide 5'-AGTACT-3'. The cleavage site was determined by comparing the ScaI-cleaved product of a primer-extended M13mp18-SCA DNA, which contains an AGTACT sequence, with dideoxy chain terminator ladders of the same DNA. ScaI was found to cleave the recognition sequence between the internal T and A, leaving flush ends to the cleaved fragments.

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Year:  1985        PMID： 2998344      PMCID： PMC1152731          DOI： 10.1042/bj2310229

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  The genome of simian virus 40.

Authors:  V B Reddy; B Thimmappaya; R Dhar; K N Subramanian; B S Zain; J Pan; P K Ghosh; M L Celma; S M Weissman
Journal:  Science       Date:  1978-05-05       Impact factor: 47.728

2.  Restriction and modification enzymes and their recognition sequences.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1985       Impact factor: 16.971

3.  Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

Authors:  J G Sutcliffe
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1979

4.  Fine cleavage map of a small colicin E1 plasmid carrying genes responsible for replication and colicin E1 immunity.

Authors:  A Oka
Journal:  J Bacteriol       Date:  1978-02       Impact factor: 3.490

5.  A new site-specific endonuclease StuI from Streptomyces tubercidicus.

Authors:  H Shimotsu; H Takahashi; H Saito
Journal:  Gene       Date:  1980-11       Impact factor: 3.688

6.  Identification of palindromic sequences recognized by restriction endonucleases, as based on the tabularized sequencing data for seven viral and plasmid DNAs.

Authors:  C Fuchs; E C Rosenvold; A Honigman; W Szybalski
Journal:  Gene       Date:  1980-09       Impact factor: 3.688

7.  The use of thin acrylamide gels for DNA sequencing.

Authors:  F Sanger; A R Coulson
Journal:  FEBS Lett       Date:  1978-03-01       Impact factor: 4.124

8.  The nucleotide sequence of bacteriophage phiX174.

Authors:  F Sanger; A R Coulson; T Friedmann; G M Air; B G Barrell; N L Brown; J C Fiddes; C A Hutchison; P M Slocombe; M Smith
Journal:  J Mol Biol       Date:  1978-10-25       Impact factor: 5.469

9.  RsaI: a new sequence-specific endonuclease activity from Rhodopseudomonas sphaeroides.

Authors:  S P Lynn; L K Cohen; S Kaplan; J F Gardner
Journal:  J Bacteriol       Date:  1980-05       Impact factor: 3.490

10.  A new site-specific endonuclease from Streptomyces lavendulae (SlaI).

Authors:  H Takahashi; M Shimizu; H Saito; Y Ikeda; H Sugisaki
Journal:  Gene       Date:  1979-01       Impact factor: 3.688
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  3 in total

1.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971

2.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1989       Impact factor: 16.971

3.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1988       Impact factor: 16.971
  3 in total

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