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Literature DB >> 6246880

The isolation and characterization of a sequence-specific endonuclease from Anabaena subcylindrica.

Abstract

An endonuclease, AsuI, was isolated from extracts of Anabaena subcylindrica on the basis of gel-electrophoretic analysis of digests of bacteriophage-lambda DNA with the paritally purified extracts. The enzyme requires Mg2+, but no other cofactors. Endonuclease AsuI recognizes the interrupted tetranucleotide sequence: (Formula: see tex), and breaks the phosphodiester bonds indicated by the arrows to leave single-stranded trinucleotide projections at the 5'-termini of the DNA fragments.

Entities: Chemical Species

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Year: 1980 PMID： 6246880 PMCID： PMC1161269 DOI： 10.1042/bj1850059

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

1. Two sequence-specific endonucleases from Anabaena oscillariodes.

Authors: A de Waard; C P van Beveren; M Duyvesteyn; H van Ormondt
Journal: FEBS Lett Date: 1979-05-01 Impact factor: 4.124

2. Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm.

Authors: M B Allen; D I Arnon
Journal: Plant Physiol Date: 1955-07 Impact factor: 8.340

3. Nucleotide sequence of bacteriophage phi X174 DNA.

Authors: F Sanger; G M Air; B G Barrell; N L Brown; A R Coulson; C A Fiddes; C A Hutchison; P M Slocombe; M Smith
Journal: Nature Date: 1977-02-24 Impact factor: 49.962

4. Recognition sequence of a restriction enzyme.

Authors: C H Bigger; K Murray; N E Murray
Journal: Nat New Biol Date: 1973-07-04

5. Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes.

Authors: H O Smith; D Nathans
Journal: J Mol Biol Date: 1973-12-15 Impact factor: 5.469

6. Plasmid identification using specific endonucleases.

Authors: R Thompson; S G Hughes; P Broda
Journal: Mol Gen Genet Date: 1974

7. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.

Authors: H O Smith; K W Wilcox
Journal: J Mol Biol Date: 1970-07-28 Impact factor: 5.469

8. A second site-specific restriction endonuclease from Staphylococcus aureus.

Authors: J S Sussenbach; P H Steenbergh; J A Rost; W J van Leeuwen; J D van Embden
Journal: Nucleic Acids Res Date: 1978-04 Impact factor: 16.971

9. Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis.

Authors: K Murray; S G Hughes; J S Brown; S A Bruce
Journal: Biochem J Date: 1976-11 Impact factor: 3.857

10. Restriction and modification in B. subtilis. Nucleotide sequence recognised by restriction endonuclease R. Bsu R from strain R.

Authors: S Bron; K Murray
Journal: Mol Gen Genet Date: 1975-12-30

8 in total

The isolation and characterization of a sequence-specific endonuclease from Anabaena subcylindrica.

1. Two sequence-specific endonucleases from Anabaena oscillariodes.

2. Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm.

3. Nucleotide sequence of bacteriophage phi X174 DNA.

4. Recognition sequence of a restriction enzyme.

5. Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes.

6. Plasmid identification using specific endonucleases.

7. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.

8. A second site-specific restriction endonuclease from Staphylococcus aureus.

9. Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis.

10. Restriction and modification in B. subtilis. Nucleotide sequence recognised by restriction endonuclease R. Bsu R from strain R.

1. Restriction enzymes and their isoschizomers.

2. Restriction enzymes and their isoschizomers.

3. Restriction and modification enzymes and their recognition sequences.

4. Restriction enzymes and their isoschizomers.

Review 5. Restriction and modification enzymes and their recognition sequences.

6. Restriction and modification enzymes and their recognition sequences.

7. Restriction and modification enzymes and their recognition sequences.

8. The nucleotide sequences recognized by endonucleases AvaI and AvaII from Anabaena variabilis.