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Literature DB >> 11780

Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis.

K Murray, S G Hughes, J S Brown, S A Bruce.

Abstract

Two endonucleases, AvaI and AvaII, were isolated from Anabaena variabilis on the basis of their ability to make a limited number of breaks at specific points in bacteriophage lambda DNA. Neither enzyme has cofactor requirements beyond Mg2+. Endonuclease AvaI makes eight breaks in the phage lambda chromosome at which the 5'-terminal sequence is pPy-C-G-N. AvaII endonuclease cuts phage lambda DNA more extensively, yielding fragments with the 5'-terminal sequence G-T-C-N or G-A-C-N. Neither enzyme generates cohesive ends.

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Year: 1976 PMID： 11780 PMCID： PMC1164119 DOI： 10.1042/bj1590317

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

23 in total

1. The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg.

Authors: A D KAISER; D S HOGNESS
Journal: J Mol Biol Date: 1960-12 Impact factor: 5.469

Review 2. Restriction endonucleases in the analysis and restructuring of dna molecules.

Authors: D Nathans; H O Smith
Journal: Annu Rev Biochem Date: 1975 Impact factor: 23.643

3. Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm.

Authors: M B Allen; D I Arnon
Journal: Plant Physiol Date: 1955-07 Impact factor: 8.340

4. Recognition sequence of a restriction enzyme.

Authors: C H Bigger; K Murray; N E Murray
Journal: Nat New Biol Date: 1973-07-04

Review 5. The primary structure of DNA.

Authors: K Murray; R W Old
Journal: Prog Nucleic Acid Res Mol Biol Date: 1974

6. Nucleotide sequence analysis with polynucleotide kinase and nucleotide "mapping" methods. 5'-Terminal sequences of deoxyribonucleic acid from bacteriophages lambda and 424.

Authors: K Murray
Journal: Biochem J Date: 1973-03 Impact factor: 3.857

7. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

8. Cleavage of DNA by R 1 restriction endonuclease generates cohesive ends.

Authors: J E Mertz; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1972-11 Impact factor: 11.205

9. An improved method for transferring nucleotides from electrophoresis strips to thin layers of ion-exchange cellulose.

Authors: E M Southern
Journal: Anal Biochem Date: 1974-11 Impact factor: 3.365

10. The behaviour of oligodeoxynucleotides on thin-layer chromatography on polyethyleneimine-cellulose and ion-exchange paper electrophoresis. Applications in fractionating and sequencing terminally labelled oligodeoxynucleotides.

Authors: A Morrison; K Murray
Journal: Biochem J Date: 1974-08 Impact factor: 3.857

24 in total

Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis.

1. The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg.

Review 2. Restriction endonucleases in the analysis and restructuring of dna molecules.

3. Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm.

4. Recognition sequence of a restriction enzyme.

Review 5. The primary structure of DNA.

6. Nucleotide sequence analysis with polynucleotide kinase and nucleotide "mapping" methods. 5'-Terminal sequences of deoxyribonucleic acid from bacteriophages lambda and 424.

7. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

8. Cleavage of DNA by R 1 restriction endonuclease generates cohesive ends.

9. An improved method for transferring nucleotides from electrophoresis strips to thin layers of ion-exchange cellulose.

10. The behaviour of oligodeoxynucleotides on thin-layer chromatography on polyethyleneimine-cellulose and ion-exchange paper electrophoresis. Applications in fractionating and sequencing terminally labelled oligodeoxynucleotides.

1. The isolation and characterization of a sequence-specific endonuclease from Anabaena subcylindrica.

2. Restriction enzymes and their isoschizomers.

3. Mapping of restriction sites in the attachment site region of bacteriophage lambda.

4. Restriction enzymes and their isoschizomers.

5. Restriction and modification enzymes and their recognition sequences.

6. A map of the cleavage sites for endonuclease AvaI in the chromosome of bacteriophage lambda.

7. The araC regulatory gene mRNA contains a leader sequence.

8. Restriction enzymes and their isoschizomers.

9. RsrII--a novel restriction endonuclease with a heptanucleotide recognition site.

10. Characterization of a restriction barrier and electrotransformation of the cyanobacterium Nostoc PCC 7121.