Literature DB >> 6243903

Large-scale purification of Japanese encephalitis virus from infected mouse brain for preparation of vaccine.

C Aizawa, S Hasegawa, C Chih-Yuan, I Yoshioka.   

Abstract

Large volumes of Japanese encephalitis (JE) virus propagated in mouse brain can be easily purified by polyethylene glycol 6,000. By using the polyethylene glycol precipitation method, mouse hemoglobin was almost all separated from the viral suspension, and consequently the total amount of nonviral protein in the viral suspension decreased. The recovery of infectivity was about 100%. The removal of residual polyethylene glycol in the viral suspension was possible without difficulty by means of ethanol precipitation. This method is recommended as an initial step in large-scale purification of Japanese encephalitis virus propagated in mouse brain because it is simple, rapid, and inexpensive.

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Year:  1980        PMID： 6243903      PMCID： PMC291283          DOI： 10.1128/aem.39.1.54-57.1980

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  15 in total

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6.  Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses.

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Authors:  Shumpei Watanabe; Tsutomu Omatsu; Mary E G Miranda; Joseph S Masangkay; Naoya Ueda; Maiko Endo; Kentaro Kato; Yukinobu Tohya; Yasuhiro Yoshikawa; Hiroomi Akashi
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  5 in total

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