Literature DB >> 6237157

Calcium-activated, phospholipid-dependent protein kinase in pig epidermis.

H Koizumi, K Adachi, M Tsutsui, S Itami, H Katayama, K M Halprin.   

Abstract

We investigated calcium-activated, phospholipid-dependent protein kinase in pig epidermis. Pig epidermal homogenates were centrifuged at 30,000 g for 30 min, and the supernatant was applied on a DEAE-cellulose column for purification. The partially purified enzyme was stimulated by simultaneous addition of Ca2+ and phospholipid. Successive addition of small amounts of diolein further activated the enzyme activity. The calcium-activated phospholipid-dependent protein kinase preferentially phosphorylated serine residues and its endogenous substrate protein in the pig epidermis has a molecular weight of about 97,000.

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Year:  1984        PMID： 6237157     DOI： 10.1111/1523-1747.ep12340330

Source DB:  PubMed          Journal:  J Invest Dermatol        ISSN： 0022-202X            Impact factor:   8.551


  4 in total

1.  Adenylate cyclase induces intracellular Ca2+ increase in single human epidermal keratinocytes of the epidermal sheet as measured by digital imaging microscopy using Fura 2-AM.

Authors:  Y Osawa; H Koizumi; T Fukaya; C Yasui; A Ohkawara; T Ueda
Journal:  Arch Dermatol Res       Date:  1991       Impact factor: 3.017

2.  Effect of hexadecane on protein kinase C of pig epidermis.

Authors:  H Koizumi; K Yamanaka; A Ohkawara
Journal:  Arch Dermatol Res       Date:  1987       Impact factor: 3.017

3.  Effects of 12-o-tetradecanoylphorbol-13-acetate and polyprenoic acid derivative on calcium-activated phospholipid-dependent protein kinase of pig skin.

Authors:  H Koizumi; H Suya; O Nemoto; T Aoyagi; A Ohkawara
Journal:  Arch Dermatol Res       Date:  1986       Impact factor: 3.017

4.  Phosphorylation of soluble pig epidermal proteins by endogenous calcium-activated, phospholipid-dependent protein kinase.

Authors:  H Koizumi; T Aoyagi; A Ohkawara
Journal:  Arch Dermatol Res       Date:  1986       Impact factor: 3.017
  4 in total

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