Literature DB >> 6227866

T4 DNA ligase can seal a nick in double-stranded DNA limited by a 5'-phosphorylated base-free deoxyribose residue.

C Goffin, W G Verly.   

Abstract

The 5' AP endodeoxyribonucleases hydrolyze the phosphodiester bond 5' to AP (apurinic or apyrimidinic) sites in double-stranded DNA leaving 3'-OH and 5'-phosphate ends. These nicks are sealed by T4 DNA ligase although the 5'-phosphate end belongs to a base-free deoxyribose.

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Year:  1983        PMID： 6227866      PMCID： PMC326563          DOI： 10.1093/nar/11.22.8103

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  Determination of single-strand breaks in DNA using neutral sucrose gradients.

Authors:  P Crine; W G Verly
Journal:  Anal Biochem       Date:  1976-10       Impact factor: 3.365

2.  Endonuclease II of Escherichia coli is exonuclease III.

Authors:  B Weiss
Journal:  J Biol Chem       Date:  1976-04-10       Impact factor: 5.157

3.  Properties of the main endonuclease specific for apurinic sites of Escherichia coli (endonuclease VI). Mechanism of apurinic site excision from DNA.

Authors:  F Gossard; W G Verly
Journal:  Eur J Biochem       Date:  1978-01-16

4.  In vitro repair of apurinic sites in DNA.

Authors:  W G Verly; F Gossard; P Crine
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205

5.  A DNase for apurinic/apyrimidinic sites associated with exonuclease III of Hemophilus influenzae.

Authors:  J E Clements; S G Rogers; B Weiss
Journal:  J Biol Chem       Date:  1978-05-10       Impact factor: 5.157

6.  Excision of apurinic sites from DNA with enzymes isolated from rat-liver chromatin.

Authors:  C Goffin; W G Verly
Journal:  Eur J Biochem       Date:  1982-10

7.  Further characterization of human fibroblast apurinic/apyrimidinic DNA endonucleases. The definition of two mechanistic classes of enzyme.

Authors:  D W Mosbaugh; S Linn
Journal:  J Biol Chem       Date:  1980-12-25       Impact factor: 5.157

8.  The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis.

Authors:  R A Laskey
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

9.  The apurinic/apyrimidinic endodeoxyribonuclease of rat-liver chromatin.

Authors:  R César; W G Verly
Journal:  Eur J Biochem       Date:  1983-01-01
  9 in total
  3 in total

1.  Endonuclease IV (nfo) mutant of Escherichia coli.

Authors:  R P Cunningham; S M Saporito; S G Spitzer; B Weiss
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

2.  Nicks 3' or 5' to AP sites or to mispaired bases, and one-nucleotide gaps can be sealed by T4 DNA ligase.

Authors:  C Goffin; V Bailly; W G Verly
Journal:  Nucleic Acids Res       Date:  1987-11-11       Impact factor: 16.971

3.  Ultrastructural characterizations of DNA nanotubes using scanning tunneling and atomic force microscopes.

Authors:  Adele Rafati; Pooria Gill
Journal:  J Microsc Ultrastruct       Date:  2015-09-28
  3 in total

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