Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Characterization and developmental expression of beta tubulin genes in Drosophila melanogaster.

Literature DB >> 6210197

Characterization and developmental expression of beta tubulin genes in Drosophila melanogaster.

S Bialojan, D Falkenburg, R Renkawitz-Pohl.

Abstract

Genomic clones containing beta tubulin sequences were isolated from a lambda library of Drosophila melanogaster. In situ hybridization localized three genes to 56D and 60B on chromosome 2 as well as to 85D on chromosome 3. The latter was known through genetic analysis to be specifically expressed during spermatogenesis. The genomic clone, pTu85, derived from this region contains one complete beta tubulin coding region as well as the 3' end of an additional so far unidentified beta tubulin gene. Genomic Southern hybridizations reveal a total of five fragments with beta tubulin homology. Clone pTu56 codes for an RNA of 1.8 kb which is expressed in all developmental stages. Clone pTu60 codes for a 2.5-kb RNA expressed during embryogenesis and pupation. In testes RNA we detected a 2.2-kb message homologous to pTu85.

Entities: Chemical

Mesh：

Substances：

Year: 1984 PMID： 6210197 PMCID： PMC557726 DOI： 10.1002/j.1460-2075.1984.tb02170.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

25 in total

1. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors: W D Benton; R W Davis
Journal: Science Date: 1977-04-08 Impact factor: 47.728

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

3. Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.

Authors: D W Cleveland; M A Lopata; R J MacDonald; N J Cowan; W J Rutter; M W Kirschner
Journal: Cell Date: 1980-05 Impact factor: 41.582

4. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

5. Mutation in a structural gene for a beta-tubulin specific to testis in Drosophila melanogaster.

Authors: K J Kemphues; R A Raff; T C Kaufman; E C Raff
Journal: Proc Natl Acad Sci U S A Date: 1979-08 Impact factor: 11.205

6. Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo.

Authors: N F Neff; J H Thomas; P Grisafi; D Botstein
Journal: Cell Date: 1983-05 Impact factor: 41.582

7. Rat insulin genes: construction of plasmids containing the coding sequences.

Authors: A Ullrich; J Shine; J Chirgwin; R Pictet; E Tischer; W J Rutter; H M Goodman
Journal: Science Date: 1977-06-17 Impact factor: 47.728

8. Characterization of cloned ribosomal DNA from Drosophila hydei.

Authors: R Renkawitz-Pohl; K H Glätzer; W Kunz
Journal: Nucleic Acids Res Date: 1980-10-24 Impact factor: 16.971

9. Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.

Authors: D A Goldberg
Journal: Proc Natl Acad Sci U S A Date: 1980-10 Impact factor: 11.205

10. Mutation in a testis-specific beta-tubulin in Drosophila: analysis of its effects on meiosis and map location of the gene.

Authors: K J Kemphues; E C Raff; R A Raff; T C Kaufman
Journal: Cell Date: 1980-09 Impact factor: 41.582

43 in total

1. Mated Drosophila melanogaster females require a seminal fluid protein, Acp36DE, to store sperm efficiently.

Authors: D M Neubaum; M F Wolfner
Journal: Genetics Date: 1999-10 Impact factor: 4.562

2. The Drosophila nuclear protein Bx42, which is found in many puffs on polytene chromosomes, is highly charged.

Authors: C Wieland; S Mann; H von Besser; H Saumweber
Journal: Chromosoma Date: 1992-06 Impact factor: 4.316

3. Naturally occurring variation in copia expression is due to both element (cis) and host (trans) regulatory variation.

Authors: L V Matyunina; I K Jordan; J F McDonald
Journal: Proc Natl Acad Sci U S A Date: 1996-07-09 Impact factor: 11.205

4. Intron and upstream sequences regulate expression of the Drosophila beta 3-tubulin gene in the visceral and somatic musculature, respectively.

Authors: A Gasch; U Hinz; R Renkawitz-Pohl
Journal: Proc Natl Acad Sci U S A Date: 1989-05 Impact factor: 11.205

5. Further sequence requirements for male germ cell-specific expression under the control of the 14 bp promoter element (beta 2UE1) of the Drosophila beta 2 tubulin gene.

Authors: F Michiels; A Wolk; R Renkawitz-Pohl
Journal: Nucleic Acids Res Date: 1991-08-25 Impact factor: 16.971

6. Characterization of a sex-influenced modifier of gene expression and suppressor of position-effect variegation in Drosophila.

Authors: U Bhadra; J A Birchler
Journal: Mol Gen Genet Date: 1996-03-20

7. Direct cloning of DNA that interacts in vivo with a specific protein: application to RNA polymerase II and sites of pausing in Drosophila.

Authors: A Law; K Hirayoshi; T O'Brien; J T Lis
Journal: Nucleic Acids Res Date: 1998-02-15 Impact factor: 16.971

8. Regulatory elements in the first intron contribute to transcriptional regulation of the beta 3 tubulin gene by 20-hydroxyecdysone in Drosophila Kc cells.

Authors: A Bruhat; S Tourmente; S Chapel; M L Sobrier; J L Couderc; B Dastugue
Journal: Nucleic Acids Res Date: 1990-05-25 Impact factor: 16.971

9. Characterization of a putative transcription factor gene expressed in the 20-OH-ecdysone inducible puff 74EF in Drosophila melanogaster.

Authors: R Janknecht; W Taube; H J Lüdecke; O Pongs
Journal: Nucleic Acids Res Date: 1989-06-26 Impact factor: 16.971

10. A member of the steroid hormone receptor gene family is expressed in the 20-OH-ecdysone inducible puff 75B in Drosophila melanogaster.

Authors: G Feigl; M Gram; O Pongs
Journal: Nucleic Acids Res Date: 1989-09-25 Impact factor: 16.971