Literature DB >> 6207637

Polyester wax embedding and sectioning technique for immunohistochemistry.

M Kusakabe, T Sakakura, Y Nishizuka, M Sano, A Matsukage.   

Abstract

We have developed a method useful for immunohistochemical studies by combining tissue fixation with buffered neutral formalin and polyester wax embedding. Buffered neutral formalin fixation preserves cell and tissue fine structure, and also the antigenicity of unstable enzymes. Polyester wax embedding makes possible thin serial sections of various tissues and preserves antigenicities for at least 6 months. We have demonstrated using this technique the localization of alpha-amylase in mouse salivary gland, parietal-cell specific antigen in mouse glandular stomach, and DNA polymerase alpha and beta in chick tissue.

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Year:  1984        PMID： 6207637     DOI： 10.3109/10520298409113845

Source DB:  PubMed          Journal:  Stain Technol        ISSN： 0038-9153


  10 in total

1.  Changes in mesenchymal cell-shape, matrix collagen and tenascin accompany bud formation in the early chick lung.

Authors:  L A Abbott; S M Lester; C A Erickson
Journal:  Anat Embryol (Berl)       Date:  1991

2.  Polyester wax: a new embedding medium for the histopathologic study of human temporal bones.

Authors:  Saumil N Merchant; Barbara Burgess; Jennifer O'Malley; Diane Jones; Joe C Adams
Journal:  Laryngoscope       Date:  2006-02       Impact factor: 3.325

3.  Tenascin is a stromal marker for epithelial malignancy in the mammary gland.

Authors:  E J Mackie; R Chiquet-Ehrismann; C A Pearson; Y Inaguma; K Taya; Y Kawarada; T Sakakura
Journal:  Proc Natl Acad Sci U S A       Date:  1987-07       Impact factor: 11.205

4.  Immunoblot analysis of the circulating antigens occurring in serum of rats infected with Angiostrongylus cantonensis.

Authors:  T Fujii
Journal:  Parasitol Res       Date:  1988       Impact factor: 2.289

5.  Characterization of a 180 kDa molecule apparently reactive with recombinant L-selectin.

Authors:  H Kawashima; N Watanabe; Y F Li; M Hirose; M Miyasaka
Journal:  Glycoconj J       Date:  1997-04       Impact factor: 2.916

6.  Angiostrongylus cantonensis: immunoblot analysis of the antigens recognized by rats.

Authors:  T Fujii
Journal:  Parasitol Res       Date:  1987       Impact factor: 2.289

7.  Immunocytochemical detection of ricin. II. Further studies using the immunoperoxidase method.

Authors:  G D Griffiths; H V Newman; D J Gee
Journal:  Histochem J       Date:  1986-04

8.  Unique distribution of the extracellular matrix component thrombospondin in the developing mouse embryo.

Authors:  K S O'Shea; V M Dixit
Journal:  J Cell Biol       Date:  1988-12       Impact factor: 10.539

9.  The hyaluronate receptor is preferentially expressed on proliferating epithelial cells.

Authors:  A M Alho; C B Underhill
Journal:  J Cell Biol       Date:  1989-04       Impact factor: 10.539

10.  A novel methodology for analysis of cell distribution in chimeric mouse organs using a strain specific antibody.

Authors:  M Kusakabe; M Yokoyama; T Sakakura; T Nomura; H L Hosick; Y Nishizuka
Journal:  J Cell Biol       Date:  1988-07       Impact factor: 10.539
  10 in total

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