Monoclonal antibody to murine neutrophils: identification of the Gm-2.2 specificity.

A neutrophil-specific alloantigen (Gm-2.2) was defined by a monoclonal antibody, 5119-4/7. The Gm-2.2 antigen is found only on bone marrow neutrophils or calcium caseinate-induced neutrophils and is absent from all lymphoid cells examined as well as adherent thioglycollate-induced peritoneal exudate cells and the nonhemopoietic tissues, kidney, liver, heart, brain, and red blood cells. Furthermore, unlike mature neutrophils, granulocyte/macrophage progenitor cells are Gm-2.2-, suggesting that Gm-2.2 is a differentiation antigen for the neutrophil series. The Gm-2 locus is linked to, but distinct from, the Ly-6 locus.