Literature DB >> 6206906

A monoclonal antibody to hemoglobin F.

H Muensch, T Hecht, K J Winkler, L Gaidulis, M Henke, K G Blume.   

Abstract

A monoclonal antibody BMU7-17 which recognizes an antigenic site unique to the cyanogen bromide fragment, CB2 (residues 56 through 133) of the gamma-chain of human hemoglobin has been produced using cell hybridization techniques. The antibody does not crossreact with hemoglobin A, the isolated alpha- or beta-chains, or with hemoglobin of various mammals. Affinity chromatography on Staphylococcal protein A-Sepharose 4B and radial immuno-diffusion revealed the antibody to be of the mouse IgG 1 class. BMU7-17 identifies F cells in adult and in cord blood specimens, as demonstrated by an indirect immunofluorescence assay. The dissociation constant of the antibody as determined using tritiated hemoglobin F with a double antibody radioimmunoassay has a Kd = 1.2 X 10(-9) moles by Scatchard plot analysis.

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Year:  1984        PMID： 6206906     DOI： 10.1007/bf00319823

Source DB:  PubMed          Journal:  Blut        ISSN： 0006-5242


  13 in total

1.  The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins.

Authors:  A M CRESTFIELD; S MOORE; W H STEIN
Journal:  J Biol Chem       Date:  1963-02       Impact factor: 5.157

2.  Specific radioimmunochemical identification and quantitation of hemoglobins A2 and F.

Authors:  F A Garver; C S Jones; M M Baker; G Altay; B P Barton; M Gravely; T H Huisman
Journal:  Am J Hematol       Date:  1976       Impact factor: 10.047

3.  Fetal hemoglobin in mixed hemopoietic colonies (CFU-GEMM), erythroid bursts (BFU-E) and erythroid colonies (CFU-E): assessment by radioimmune assay and immunofluorescence.

Authors:  A A Fauser; H A Messner
Journal:  Blood       Date:  1979-12       Impact factor: 22.113

4.  The estimation of fetal haemoglobin in healthy adults by radioimmunoassay.

Authors:  P C Rutland; M E Pembrey; T Davies
Journal:  Br J Haematol       Date:  1983-04       Impact factor: 6.998

5.  A better cell line for making hybridomas secreting specific antibodies.

Authors:  M Shulman; C D Wilde; G Köhler
Journal:  Nature       Date:  1978-11-16       Impact factor: 49.962

6.  The G gamma:A gamma composition of fetal hemoglobin in fetuses and newborns.

Authors:  B P Alter
Journal:  Blood       Date:  1979-11       Impact factor: 22.113

7.  Abnormal human haemoglobins. Separation and characterization of the alpha and beta chains by chromatography, and the determination of two new variants, hb Chesapeak and hb J (Bangkok).

Authors:  J B Clegg; M A Naughton; D J Weatherball
Journal:  J Mol Biol       Date:  1966-08       Impact factor: 5.469

8.  Monoclonal antibodies specific for globin chains.

Authors:  G Stamatoyannopoulos; M Farquhar; D Lindsley; M Brice; T Papayannopoulou; P E Nute
Journal:  Blood       Date:  1983-03       Impact factor: 22.113

9.  The four subclasses of IgG can be isolated from mouse serum by using Protein A-Sepharose.

Authors:  I Seppälä; H Sarvas; F Péterfy; O Mäkelä
Journal:  Scand J Immunol       Date:  1981-10       Impact factor: 3.487

10.  Tritium labeling of proteins to high specific radioactivity by reduction methylation.

Authors:  B F Tack; J Dean; D Eilat; P E Lorenz; A N Schechter
Journal:  J Biol Chem       Date:  1980-09-25       Impact factor: 5.157
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  2 in total

1.  A monoclonal antibody to the heavy chain of human IgM with a particular reaction pattern in human B-cell malignancies.

Authors:  M Henke; S Santos; C D Winberg; J E Shively
Journal:  Blut       Date:  1987-09

2.  A new anti-hemoglobin F antibody against synthetic peptides for the detection of F-cell precursors (F-blasts) in bone marrow.

Authors:  J W Choi; Y Kim; M Fujino; M Ito
Journal:  Int J Hematol       Date:  2001-10       Impact factor: 2.490
  2 in total

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