Literature DB >> 6202663

Development and application of a monoclonal rat peroxidase antiperoxidase (PAP) immunocytochemical reagent.

A C Cuello, C Milstein, B Wright, S Bramwell, J V Priestley, J Jarvis.   

Abstract

Monoclonal antibodies are being increasingly used in immunocytochemistry but their localisation by the peroxidase antiperoxidase (PAP) procedure requires the use of rat or mouse PAP. In this paper we describe the development and application of a monoclonal rat PAP. This reagent has been used successfully for immunocytochemistry at light and electron microscopy level in combination with rat monoclonal antibodies against serotonin (5-HT), substance P and somatostatin. The monoclonal rat PAP has several advantages over conventional polyclonal rat PAP and is likely to be a valuable developing reagent in immunocytochemistry using rat monoclonal antibodies.

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Year:  1984        PMID： 6202663     DOI： 10.1007/bf00495774

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  12 in total

1.  Detection of substance P in the central nervous system by a monoclonal antibody.

Authors:  A C Cuello; G Galfre; C Milstein
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

2.  The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes.

Authors:  L A Sternberger; P H Hardy; J J Cuculis; H G Meyer
Journal:  J Histochem Cytochem       Date:  1970-05       Impact factor: 2.479

3.  Neurotransmitter-specific projection neurons revealed by combining PAP immunohistochemistry with retrograde transport of HRP.

Authors:  J V Priestley; P Somogyi; A C Cuello
Journal:  Brain Res       Date:  1981-09-14       Impact factor: 3.252

4.  Hybrid hybridomas and their use in immunohistochemistry.

Authors:  C Milstein; A C Cuello
Journal:  Nature       Date:  1983 Oct 6-12       Impact factor: 49.962

5.  Immunocytochemical localization of substance P in the spinal trigeminal nucleus of the rat: a light and electron microscopic study.

Authors:  J V Priestley; P Somogyi; A C Cuello
Journal:  J Comp Neurol       Date:  1982-10-10       Impact factor: 3.215

6.  Preparation of peroxidase: antiperoxidase (PAP) complexes for immunohistological labeling of monoclonal antibodies.

Authors:  D Y Mason; J L Cordell; Z Abdulaziz; M Naiem; G Bordenave
Journal:  J Histochem Cytochem       Date:  1982-11       Impact factor: 2.479

7.  Immunocytochemistry with internally labeled monoclonal antibodies.

Authors:  A C Cuello; J V Priestley; C Milstein
Journal:  Proc Natl Acad Sci U S A       Date:  1982-01       Impact factor: 11.205

8.  Preparation of monoclonal antibodies: strategies and procedures.

Authors:  G Galfrè; C Milstein
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

9.  Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG.

Authors:  G Galfrè; C Milstein; B Wright
Journal:  Nature       Date:  1979-01-11       Impact factor: 49.962

10.  Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.

Authors:  J V Kilmartin; B Wright; C Milstein
Journal:  J Cell Biol       Date:  1982-06       Impact factor: 10.539
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  6 in total

1.  Production of a bi-specific monoclonal antibody recognizing mouse kappa light chains and horseradish peroxidase. Applications in immunoassays.

Authors:  R L Kenigsberg; A C Cuello
Journal:  Histochemistry       Date:  1990

2.  Advantages of bispecific hybridomas in one-step immunocytochemistry and immunoassays.

Authors:  M R Suresh; A C Cuello; C Milstein
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

3.  Reorganization of cholinergic terminals in the cerebral cortex and hippocampus in transgenic mice carrying mutated presenilin-1 and amyloid precursor protein transgenes.

Authors:  T P Wong; T Debeir; K Duff; A C Cuello
Journal:  J Neurosci       Date:  1999-04-01       Impact factor: 6.167

4.  Ethylcholine mustard aziridinium ion lesions of the rat cortex result in retrograde degeneration of basal forebrain cholinergic neurons: implications for animal models of neurodegenerative disease.

Authors:  P H Stephens; P Tagari; A C Cuello
Journal:  Neurochem Res       Date:  1987-07       Impact factor: 3.996

Review 5.  Antibodies as molecular probes in neurobiology. Identification of chemically defined neurons and synapses in tissues and tissue cultures.

Authors:  J Pecci Saavedra; A Brusco; J J López-Costa; L A Gómez; E M López
Journal:  Mol Neurobiol       Date:  1992       Impact factor: 5.590

6.  Development of a mouse antiperoxidase secreting hybridoma for use in the production of a mouse PAP complex for immunocytochemistry and as a parent cell line in the development of hybrid hybridomas.

Authors:  F M Semenenko; S Bramwell; E Sidebottom; A C Cuello
Journal:  Histochemistry       Date:  1985
  6 in total

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