Literature DB >> 6183312

Preparation of peroxidase: antiperoxidase (PAP) complexes for immunohistological labeling of monoclonal antibodies.

D Y Mason, J L Cordell, Z Abdulaziz, M Naiem, G Bordenave.   

Abstract

The production of mouse peroxidase:antiperoxidase (PAP) complexes suitable for immunohistological use in conjunction with monoclonal antibodies is described. Three approaches were explored: 1) production of conventional polyclonal PAP complexes; 2) conversion of rabbit PAP to "pseudo-mouse PAP" by incubation with monoclonal mouse anti-rabbit immunoglobulin; 3) formation of PAP complexes from monoclonal mouse antiperoxidase. PAP complexes prepared by the latter technique gave the best immunohistological labeling reactions, being stable on storage and compatible with a wide range of human monoclonal antibodies. Gel filtration revealed that monoclonal PAP is of lower molecular weight than conventional PAP complexes (fulfilling theoretical predictions based on the monospecificity of monoclonal antibodies).

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 6183312     DOI： 10.1177/30.11.6183312

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  24 in total

1.  Behaviour of chick embryo aortic cells obtained through nonenzymatic means cultured onto collagen gels.

Authors:  E A Arciniegas; M A Mota; M C Castillo
Journal:  Anat Embryol (Berl)       Date:  1990

2.  Production of a bi-specific monoclonal antibody recognizing mouse kappa light chains and horseradish peroxidase. Applications in immunoassays.

Authors:  R L Kenigsberg; A C Cuello
Journal:  Histochemistry       Date:  1990

3.  Characterisation of normal peripheral blood cells in cycle identified by monoclonal antibody Ki-67.

Authors:  I Cordone; E Matutes; D Catovsky
Journal:  J Clin Pathol       Date:  1992-03       Impact factor: 3.411

4.  Accumulation of p27(kip1) is associated with cyclin D3 overexpression in the oxyphilic (Hurthle cell) variant of follicular thyroid carcinoma.

Authors:  G Troncone; A Iaccarino; M Russo; E A Palmieri; M Volante; M Papotti; G Viglietto; L Palombini
Journal:  J Clin Pathol       Date:  2006-06-23       Impact factor: 3.411

5.  Platelet satellitism and alpha granule proteins.

Authors:  C Christopoulos; C Mattock
Journal:  J Clin Pathol       Date:  1991-09       Impact factor: 3.411

6.  Increased production of interleukin 1 beta and hepatocyte growth factor may contribute to foveolar hyperplasia in enlarged fold gastritis.

Authors:  Y Yasunaga; Y Shinomura; S Kanayama; Y Higashimoto; M Yabu; Y Miyazaki; S Kondo; Y Murayama; H Nishibayashi; S Kitamura; Y Matsuzawa
Journal:  Gut       Date:  1996-12       Impact factor: 23.059

7.  A 90-kd surface antigen from a subpopulation of smooth muscle cells from human atherosclerotic lesions.

Authors:  O J Printseva; M M Peclo; A V Tjurmin; A I Faerman; S M Danilov; V S Repin; V N Smirnov
Journal:  Am J Pathol       Date:  1989-02       Impact factor: 4.307

8.  Monoclonal glucose-oxidase-anti-glucose-oxidase (GAG) immunosandwich assay for the detection of monoclonal antibodies on routine hematological smears.

Authors:  M Pfreundschuh; M Lehmann; T Steinmetz; H H Kirchner; V Diehl
Journal:  Blut       Date:  1988-03

9.  Monoclonal antibodies PG-B6a and PG-B6p recognize, respectively, a highly conserved and a formol-resistant epitope on the human BCL-6 protein amino-terminal region.

Authors:  L Flenghi; B Bigerna; M Fizzotti; S Venturi; L Pasqualucci; S Pileri; B H Ye; M Gambacorta; R Pacini; C D Baroni; E Pescarmona; I Anagnostopoulos; H Stein; G Asdrubali; M F Martelli; P G Pelicci; R Dalla-Favera; B Falini
Journal:  Am J Pathol       Date:  1996-05       Impact factor: 4.307

10.  Development of an improved animal model of shigellosis in the adult rabbit by colonic infection with Shigella flexneri 2a.

Authors:  G H Rabbani; M J Albert; H Rahman; M Islam; D Mahalanabis; I Kabir; K Alam; M Ansaruzzaman
Journal:  Infect Immun       Date:  1995-11       Impact factor: 3.441
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.