Literature DB >> 6202168

Detection of protein in polyacrylamide gels using an improved silver stain.

T Marshall.   

Abstract

A much improved silver staining procedure for the detection of protein in polyacrylamide gels of 0.8-3.0 mm thickness is described. It achieves very high sensitivity (detecting less than 0.01 ng bovine serum albumin/mm2) by overstaining and subsequently removing nonspecific background stain using a modified, reliable destaining procedure. Maximum sensitivity follows prediamine equilibration in 0.1% (w/v) formaldehyde solution. With two-dimensional electrophoresis the improved staining procedure reveals greater than 200 polypeptides in unconcentrated human urine and greater than 150 polypeptides in a single human fingerprint.

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Year:  1984        PMID： 6202168     DOI： 10.1016/0003-2697(84)90227-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  11 in total

1.  Immunogenic activity of gonococcal protein I in mice with three different lipoidal adjuvants delivered in liposomes and in complexes.

Authors:  W Jiskoot; T Teerlink; M M Van Hoof; K Bartels; V Kanhai; D J Crommelin; E C Beuvery
Journal:  Infect Immun       Date:  1986-11       Impact factor: 3.441

2.  Proteins of the extracellular matrix in vitreoretinal membranes.

Authors:  O F Scheiffarth; A Kampik; H Günther; K von der Mark
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  1988       Impact factor: 3.117

3.  A DNA helicase induced by herpes simplex virus type 1.

Authors:  J J Crute; E S Mocarski; I R Lehman
Journal:  Nucleic Acids Res       Date:  1988-07-25       Impact factor: 16.971

4.  Identification of immunosuppressive fractions from the rat submandibular salivary gland.

Authors:  M Abdelhaleem; E Sabbadini
Journal:  Immunology       Date:  1992-06       Impact factor: 7.397

5.  Herpes simplex virus 1 helicase-primase: a complex of three herpes-encoded gene products.

Authors:  J J Crute; T Tsurumi; L A Zhu; S K Weller; P D Olivo; M D Challberg; E S Mocarski; I R Lehman
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

6.  Further biochemical characterization of wheat DNA primase: possible functional implication of copurification with DNA polymerase A.

Authors:  P Laquel; M Castroviejo; S Litvak
Journal:  Nucleic Acids Res       Date:  1990-08-25       Impact factor: 16.971

7.  A host cell protein binds to a highly conserved sequence element (pac-2) within the cytomegalovirus a sequence.

Authors:  G W Kemble; E S Mocarski
Journal:  J Virol       Date:  1989-11       Impact factor: 5.103

8.  Association of DNA helicase and primase activities with a subassembly of the herpes simplex virus 1 helicase-primase composed of the UL5 and UL52 gene products.

Authors:  M S Dodson; I R Lehman
Journal:  Proc Natl Acad Sci U S A       Date:  1991-02-15       Impact factor: 11.205

9.  Purification, immunochemical quantification and localization in rat heart of putative fatty acid translocase (FAT/CD36).

Authors:  Joep F F Brinkmann; Maurice M A L Pelsers; Frans A van Nieuwenhoven; Narendra N Tandon; Ger J van der Vusse; Jan F C Glatz
Journal:  Mol Cell Biochem       Date:  2006-03-16       Impact factor: 3.396

10.  Chalcone synthases from spinach (Spinacia oleracea L.) : I. Purification, peptide patterns, and immunological properties of different forms.

Authors:  L Beerhues; R Wiermann
Journal:  Planta       Date:  1988-12       Impact factor: 4.116
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