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Literature DB >> 1847509

Association of DNA helicase and primase activities with a subassembly of the herpes simplex virus 1 helicase-primase composed of the UL5 and UL52 gene products.

Abstract

Herpes simplex virus 1 encodes a helicase-primase that is composed of the products of the UL5, UL8, and UL52 genes. A stable subassembly consisting of only the UL5 and UL52 gene products has been purified to near homogeneity from insect cells doubly infected with baculovirus recombinant for these two genes. The purified subassembly has the DNA-dependent ATPase, DNA-dependent GTPase, DNA helicase, and DNA primase activities that are characteristic of the three-subunit holoenzyme. The purified UL8 gene product, although required for viral DNA replication, neither exhibits these enzymatic activities nor stably associates with either the UL5 or the UL52 gene product.

Entities: Gene Species

Mesh：

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Year: 1991 PMID： 1847509 PMCID： PMC50965 DOI： 10.1073/pnas.88.4.1105

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

17 in total

1. Overexpression and assembly of the herpes simplex virus type 1 helicase-primase in insect cells.

Authors: M S Dodson; J J Crute; R C Bruckner; I R Lehman
Journal: J Biol Chem Date: 1989-12-15 Impact factor: 5.157

2. Identification of herpes simplex virus type 1 genes required for origin-dependent DNA synthesis.

Authors: C A Wu; N J Nelson; D J McGeoch; M D Challberg
Journal: J Virol Date: 1988-02 Impact factor: 5.103

Review 3. Animal virus DNA replication.

Authors: M D Challberg; T J Kelly
Journal: Annu Rev Biochem Date: 1989 Impact factor: 23.643

4. A new superfamily of replicative proteins.

Authors: T C Hodgman
Journal: Nature Date: 1988-05-05 Impact factor: 49.962

5. A DNA helicase induced by herpes simplex virus type 1.

Authors: J J Crute; E S Mocarski; I R Lehman
Journal: Nucleic Acids Res Date: 1988-07-25 Impact factor: 16.971

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

7. Herpes simplex virus type 1 gene products required for DNA replication: identification and overexpression.

Authors: P D Olivo; N J Nelson; M D Challberg
Journal: J Virol Date: 1989-01 Impact factor: 5.103

8. Herpes simplex virus 1 helicase-primase: a complex of three herpes-encoded gene products.

Authors: J J Crute; T Tsurumi; L A Zhu; S K Weller; P D Olivo; M D Challberg; E S Mocarski; I R Lehman
Journal: Proc Natl Acad Sci U S A Date: 1989-04 Impact factor: 11.205

9. Herpes simplex virus type 1 DNA synthesis requires the product of the UL8 gene: isolation and characterization of an ICP6::lacZ insertion mutation.

Authors: E P Carmichael; S K Weller
Journal: J Virol Date: 1989-02 Impact factor: 5.103

10. Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes.

Authors: A E Gorbalenya; E V Koonin; A P Donchenko; V M Blinov
Journal: Nucleic Acids Res Date: 1989-06-26 Impact factor: 16.971

41 in total

1. The UL8 subunit of the herpes simplex virus helicase-primase complex is required for efficient primer utilization.

Authors: G Sherman; J Gottlieb; M D Challberg
Journal: J Virol Date: 1992-08 Impact factor: 5.103

Review 2. Eukaryotic DNA helicases: essential enzymes for DNA transactions.

Authors: P Thömmes; U Hübscher
Journal: Chromosoma Date: 1992-06 Impact factor: 4.316

Review 3. Understanding helicases as a means of virus control.

Authors: D N Frick; A M I Lam
Journal: Curr Pharm Des Date: 2006 Impact factor: 3.116

4. The six conserved helicase motifs of the UL5 gene product, a component of the herpes simplex virus type 1 helicase-primase, are essential for its function.

Authors: L A Zhu; S K Weller
Journal: J Virol Date: 1992-01 Impact factor: 5.103

5. The UL5 gene of herpes simplex virus type 1: isolation of a lacZ insertion mutant and association of the UL5 gene product with other members of the helicase-primase complex.