Literature DB >> 6197185

Conformation of promoter DNA: fine mapping of S1-hypersensitive sites.

E Schon, T Evans, J Welsh, A Efstratiadis.   

Abstract

Larsen and Weintraub showed that a feature of active (but not inactive) chromatin is the appearance of S1-nuclease-hypersensitive sites in the 5'-flanking regions of expressing genes. Such sites are also present in supercoiled, but not relaxed, plasmids carrying these gene sequences. We have developed an in vitro approach to map, at the nucleotide level, S1-hypersensitive sites in artificially generated supercoiled circles of naked DNA. In the case of the adult chicken beta-globin gene, our results correlate extremely well with the coarser in vivo mapping. These sites reside in homopurine-homopyrimidine tracts, and, despite their S1 sensitivity, they do not seem to be single-stranded.

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Year:  1983        PMID： 6197185     DOI： 10.1016/0092-8674(83)90116-2

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  70 in total

1.  Initiation of rolling-circle replication in pT181 plasmid: initiator protein enhances cruciform extrusion at the origin.

Authors:  P Noirot; J Bargonetti; R P Novick
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

2.  Diverse soybean actin transcripts contain a large intron in the 5' untranslated leader: structural similarity to vertebrate muscle actin genes.

Authors:  L Pearson; R B Meagher
Journal:  Plant Mol Biol       Date:  1990-04       Impact factor: 4.076

3.  Complex structural behavior of oligopurine-oligopyrimidine sequence cloned within the supercoiled plasmid.

Authors:  P Parniewski; G Galazka; A Wilk; J Klysik
Journal:  Nucleic Acids Res       Date:  1989-01-25       Impact factor: 16.971

4.  Reaction conditions affect the specificity of bromoacetaldehyde as a probe for DNA cruciforms and B-Z junctions.

Authors:  M J McLean; J E Larson; F Wohlrab; R D Wells
Journal:  Nucleic Acids Res       Date:  1987-09-11       Impact factor: 16.971

5.  A DNA-binding factor in adult hematopoietic cells interacts with a pyrimidine-rich domain upstream from the human delta-globin gene.

Authors:  D O'Neill; K Bornschlegel; M Flamm; M Castle; A Bank
Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-15       Impact factor: 11.205

6.  Activating transcription from single stranded DNA.

Authors:  T Tomonaga; D Levens
Journal:  Proc Natl Acad Sci U S A       Date:  1996-06-11       Impact factor: 11.205

7.  Agrobacterium T-DNA integration in Arabidopsis is correlated with DNA sequence compositions that occur frequently in gene promoter regions.

Authors:  Richard G Schneeberger; Ke Zhang; Tatiana Tatarinova; Max Troukhan; Shing F Kwok; Josh Drais; Kevin Klinger; Francis Orejudos; Kimberly Macy; Amit Bhakta; James Burns; Gopal Subramanian; Jonathan Donson; Richard Flavell; Kenneth A Feldmann
Journal:  Funct Integr Genomics       Date:  2005-03-03       Impact factor: 3.410

8.  Defining a bacteriophage T4 late promoter: bacteriophage T4 gene 55 protein suffices for directing late promoter recognition.

Authors:  G A Kassavetis; E P Geiduschek
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205

9.  Conformational DNA transition in the in vitro torsionally strained chicken beta-globin 5' region.

Authors:  L Runkel; A Nordheim
Journal:  Nucleic Acids Res       Date:  1986-09-25       Impact factor: 16.971

10.  Cloning of two novel forms of human acidic fibroblast growth factor (aFGF) mRNA.

Authors:  R A Payson; H Canatan; M A Chotani; W P Wang; S E Harris; R L Myers; I M Chiu
Journal:  Nucleic Acids Res       Date:  1993-02-11       Impact factor: 16.971
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