| Literature DB >> 2644622 |
P Parniewski1, G Galazka, A Wilk, J Klysik.
Abstract
Synthetic sequence GATCC(AG)7ATCG(AT)4CG(AG)7 was cloned into plasmid and its structural behavior under the influence of supercoiling was analysed by chemical modification at variety of experimental conditions. It was found that this sequence adopts at least two different non-B conformations depending on -delta and pH values. Moreover, 12 nucleotide long non-pur.pyr spacer region separating two identical (AG)7 blocks does not provide a significant energy barrier protecting against unusual structures formation.Entities:
Mesh:
Substances:
Year: 1989 PMID: 2644622 PMCID: PMC331607 DOI: 10.1093/nar/17.2.617
Source DB: PubMed Journal: Nucleic Acids Res ISSN: 0305-1048 Impact factor: 16.971