Literature DB >> 6192499

Expression of streptococcal M protein in Escherichia coli.

J R Scott, V A Fischetti.   

Abstract

The structural gene for group A streptococcal M protein, the fibrillar surface molecule enabling the organism to resist phagocytosis, has been cloned into Escherichia coli. The molecule produced by Escherichia coli is slightly larger than the M protein isolated by solubilization of the streptococcal cell wall, but is similar in size to that secreted by streptococcal protoplast and L forms. Immunologically, the molecule synthesized by Escherichia coli has the same type-specific determinants as the streptococcal M protein.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6192499     DOI： 10.1126/science.6192499

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  36 in total

1.  Degenerate oligonucleotide primers for enzymatic amplification of recA sequences from gram-positive bacteria and mycoplasmas.

Authors:  K Dybvig; S K Hollingshead; D G Heath; D B Clewell; F Sun; A Woodard
Journal:  J Bacteriol       Date:  1992-04       Impact factor: 3.490

2.  The presence of conjugative transposon Tn916 in the recipient strain does not impede transfer of a second copy of the element.

Authors:  M Norgren; J R Scott
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

3.  Cell wall anchoring of the Streptococcus pyogenes M6 protein in various lactic acid bacteria.

Authors:  J C Piard; I Hautefort; V A Fischetti; S D Ehrlich; M Fons; A Gruss
Journal:  J Bacteriol       Date:  1997-05       Impact factor: 3.490

4.  Molecular epidemiologic analysis of the type A streptococcal exotoxin (erythrogenic toxin) gene (speA) in clinical Streptococcus pyogenes strains.

Authors:  C E Yu; J J Ferretti
Journal:  Infect Immun       Date:  1989-12       Impact factor: 3.441

5.  Expression of protective and cardiac tissue cross-reactive epitopes of type 5 streptococcal M protein in Escherichia coli.

Authors:  T P Poirier; M A Kehoe; J B Dale; K N Timmis; E H Beachey
Journal:  Infect Immun       Date:  1985-04       Impact factor: 3.441

6.  A method for allelic replacement that uses the conjugative transposon Tn916: deletion of the emm6.1 allele in Streptococcus pyogenes JRS4.

Authors:  M Norgren; M G Caparon; J R Scott
Journal:  Infect Immun       Date:  1989-12       Impact factor: 3.441

7.  Cloning and surface expression in Escherichia coli of a structural gene encoding a surface protein of Haemophilus influenzae type b.

Authors:  P L Holmans; T A Loftus; E J Hansen
Journal:  Infect Immun       Date:  1985-10       Impact factor: 3.441

8.  Expression of Actinomyces viscosus antigens in Escherichia coli: cloning of a structural gene (fimA) for type 2 fimbriae.

Authors:  J A Donkersloot; J O Cisar; M E Wax; R J Harr; B M Chassy
Journal:  J Bacteriol       Date:  1985-06       Impact factor: 3.490

9.  Streptokinase: cloning, expression, and excretion by Escherichia coli.

Authors:  H Malke; J J Ferretti
Journal:  Proc Natl Acad Sci U S A       Date:  1984-06       Impact factor: 11.205

10.  M protein mediates streptococcal adhesion to HEp-2 cells.

Authors:  J R Wang; M W Stinson
Journal:  Infect Immun       Date:  1994-02       Impact factor: 3.441
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.