Literature DB >> 6190674

Evidence for coordinated phosphorylation of keratins and vimentin during mitosis in transformed human amnion cells. Phosphate turnover of modified proteins.

S J Fey, P M Larsen, J E Celis.   

Abstract

Two-dimensional gel electrophoresis (IEF) analysis of short-term [32P]orthophosphate-labelled intermediate-sized filament proteins (keratins and vimentin) from transformed mitotic amnion cells (AMA), have shown that these proteins are modified coordinately and that the half life of the phosphate is about 13 min for the keratins and 11 min for vimentin. These results support the notion that the preferential modification of intermediate-sized filament proteins during mitosis may play a role in modulating filament associations with organelles and other cellular structures.

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Year:  1983        PMID： 6190674     DOI： 10.1016/0014-5793(83)81138-7

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  Cell cycle-dependent changes in the organization of an intermediate filament-associated protein: correlation with phosphorylation by p34cdc2.

Authors:  O Skalli; Y H Chou; R D Goldman
Journal:  Proc Natl Acad Sci U S A       Date:  1992-12-15       Impact factor: 11.205

2.  Markers for human placental trophoblasts in two-dimensional gel electrophoresis.

Authors:  Z Jie; S J Fey; H Hager; P Høllsberg; P Ebbesen; P M Larsen
Journal:  In Vitro Cell Dev Biol       Date:  1990-10

3.  Head and rod 1 interactions in vimentin: identification of contact sites, structure, and changes with phosphorylation using site-directed spin labeling and electron paramagnetic resonance.

Authors:  Atya Aziz; John F Hess; Madhu S Budamagunta; Paul G FitzGerald; John C Voss
Journal:  J Biol Chem       Date:  2008-12-31       Impact factor: 5.157

4.  Expression of the transformation-sensitive protein "cyclin" in normal human epidermal basal cells and simian virus 40-transformed keratinocytes.

Authors:  J E Celis; S J Fey; P M Larsen; A Celis
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205

5.  Intermediate filaments in monkey kidney TC7 cells: focal centers and interrelationship with other cytoskeletal systems.

Authors:  J E Celis; J V Small; P M Larsen; S J Fey; J De Mey; A Celis
Journal:  Proc Natl Acad Sci U S A       Date:  1984-02       Impact factor: 11.205

6.  Involvement of vimentin in the reverse transformation reaction.

Authors:  D Chan; A Goate; T T Puck
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

7.  Phosphorylation of vimentin in mitotically selected cells. In vitro cyclic AMP-independent kinase and calcium-stimulated phosphatase activities.

Authors:  R M Evans
Journal:  J Cell Biol       Date:  1989-01       Impact factor: 10.539

8.  Phosphorylation of keratin and vimentin polypeptides in normal and transformed mitotic human epithelial amnion cells: behavior of keratin and vimentin filaments during mitosis.

Authors:  J E Celis; P M Larsen; S J Fey; A Celis
Journal:  J Cell Biol       Date:  1983-11       Impact factor: 10.539

9.  Modulation of vimentin containing intermediate filament distribution and phosphorylation in living fibroblasts by the cAMP-dependent protein kinase.

Authors:  N J Lamb; A Fernandez; J R Feramisco; W J Welch
Journal:  J Cell Biol       Date:  1989-06       Impact factor: 10.539
  9 in total

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