Literature DB >> 6190564

Induced genetic deficiency of the nucleolar organizer in rat kangaroo cells (PTK1) by ultraviolet laser microirradiation.

H Liang, M W Berns.   

Abstract

An ultraviolet laser microbeam was used to irradiate one of the two nucleolar organizer regions of PTK1 cells in early prophase. The directed nucleolar deficiency induced by ultraviolet laser irradiation was maintained in the daughter cells through subsequent cell generations. However, the frequent occurrence of spontaneous cell fusion in low density cells following the cloning procedure facilitated a recovery of cells to two or more nucleoli.

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Year:  1983        PMID: 6190564     DOI: 10.1007/BF02788584

Source DB:  PubMed          Journal:  Cell Biophys        ISSN: 0163-4992


  8 in total

1.  Directed chromosome loss by laser microirradiation.

Authors:  M W Berns
Journal:  Science       Date:  1974-11-22       Impact factor: 47.728

2.  Laser microirradiation of chromosomes.

Authors:  M W Berns
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1974

3.  Modification of nucleolar expression following laser micro-irradiation of chromosomes.

Authors:  M W Berns; Y Ohnuki; D E Rounds; R S Olson
Journal:  Exp Cell Res       Date:  1970-04       Impact factor: 3.905

4.  Laser microirradiation of the nucleolar organizer in cells of the rat kangaroo (Potorous tridactylis). Reduction of nucleolar number and production of micronucleoli.

Authors:  M W Berns; A D Floyd; K Adkisson; W K Cheng; L Moore; G Hoover; K Ustick; S Burgott; T Osial
Journal:  Exp Cell Res       Date:  1972-12       Impact factor: 3.905

5.  Laser microbeam irradiation of the juxtanucleolar region of prophase nucleolar chromosomes.

Authors:  Y Onuki; D E Rounds; R S Olson; M W Berns
Journal:  Exp Cell Res       Date:  1972-03       Impact factor: 3.905

6.  Are chromosome secondary constrictions nucleolar organizers? A re-examination using a laser microbeam.

Authors:  M W Berns; W K Cheng
Journal:  Exp Cell Res       Date:  1971-11       Impact factor: 3.905

7.  Genetic microsurgery by laser: establishment of a clonal population of rat kangaroo cells (PTK2) with a directed deficiency in a chromosomal nucleolar organizer.

Authors:  M W Berns; L K Chong; M Hammer-Wilson; K Miller; A Siemens
Journal:  Chromosoma       Date:  1979-06-21       Impact factor: 4.316

8.  Partial cell irradiation with a tunable organic dye laser.

Authors:  M W Berns
Journal:  Nature       Date:  1972-12-22       Impact factor: 49.962

  8 in total
  1 in total

1.  Study on mechanism of micronucleoli formation by laser microirradiation.

Authors:  Y H Hu; H Liang; Y Q Jiang
Journal:  Cell Biophys       Date:  1989-06
  1 in total

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